7PBG

4-ethylphenol oxidase from Gulosibacter chungangensis: native structure

7PBG の概要

• エントリーDOI: 10.2210/pdb7pbg/pdb
• 分子名称: FAD-binding oxidoreductase, FLAVIN-ADENINE DINUCLEOTIDE, CHLORIDE ION, ... (4 entities in total)
• 機能のキーワード: flavoprotein, biocatalysis, genome mining, enzyme structure, 4-vinylphenol, dehydrogenation, 4-alkylphenol oxidase, oxidoreductase
• 由来する生物種: Gulosibacter chungangensis
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 119750.58

構造登録者

Alvigini, L.,Mattevi, A. (登録日: 2021-08-02, 公開日: 2021-09-29, 最終更新日: 2024-11-20)

主引用文献

Alvigini, L.,Gran-Scheuch, A.,Guo, Y.,Trajkovic, M.,Saifuddin, M.,Fraaije, M.W.,Mattevi, A.
Discovery, Biocatalytic Exploration and Structural Analysis of a 4-Ethylphenol Oxidase from Gulosibacter chungangensis.
Chembiochem, 22:3225-3233, 2021

PubMed Abstract: The vanillyl-alcohol oxidase (VAO) family is a rich source of biocatalysts for the oxidative bioconversion of phenolic compounds. Through genome mining and sequence comparisons, we found that several family members lack a generally conserved catalytic aspartate. This finding led us to study a VAO-homolog featuring a glutamate residue in place of the common aspartate. This 4-ethylphenol oxidase from Gulosibacter chungangensis (Gc4EO) shares 42 % sequence identity with VAO from Penicillium simplicissimum, contains the same 8α-N -histidyl-bound FAD and uses oxygen as electron acceptor. However, Gc4EO features a distinct substrate scope and product specificity as it is primarily effective in the dehydrogenation of para-substituted phenols with little generation of hydroxylated products. The three-dimensional structure shows that the characteristic glutamate side chain creates a closely packed environment that may limit water accessibility and thereby protect from hydroxylation. With its high thermal stability, well defined structural properties and high expression yields, Gc4EO may become a catalyst of choice for the specific dehydrogenation of phenolic compounds bearing small substituents.

PubMed: 34523783
DOI: 10.1002/cbic.202100457

実験手法

X-RAY DIFFRACTION (1.6 Å)