7PB9

Crystal structure of tandem WH domains of Vps25 from Odinarchaeota

7PB9 の概要

• エントリーDOI: 10.2210/pdb7pb9/pdb
• 分子名称: Tandem WH domains of Vps25 (2 entities in total)
• 機能のキーワード: escrt-ii, escrt, asgard archaea, membrane trafficking, odinarchaeota, membrane protein
• 由来する生物種: Odinarchaeota archaeon (strain LCB_4)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 22006.42

構造登録者

Salzer, R.,Bellini, D.,Papatziamou, D.,Robinson, N.P.,Lowe, J. (登録日: 2021-08-01, 公開日: 2022-06-08, 最終更新日: 2024-01-31)

主引用文献

Hatano, T.,Palani, S.,Papatziamou, D.,Salzer, R.,Souza, D.P.,Tamarit, D.,Makwana, M.,Potter, A.,Haig, A.,Xu, W.,Townsend, D.,Rochester, D.,Bellini, D.,Hussain, H.M.A.,Ettema, T.J.G.,Lowe, J.,Baum, B.,Robinson, N.P.,Balasubramanian, M.
Asgard archaea shed light on the evolutionary origins of the eukaryotic ubiquitin-ESCRT machinery.
Nat Commun, 13:3398-3398, 2022

PubMed Abstract: The ESCRT machinery, comprising of multiple proteins and subcomplexes, is crucial for membrane remodelling in eukaryotic cells, in processes that include ubiquitin-mediated multivesicular body formation, membrane repair, cytokinetic abscission, and virus exit from host cells. This ESCRT system appears to have simpler, ancient origins, since many archaeal species possess homologues of ESCRT-III and Vps4, the components that execute the final membrane scission reaction, where they have been shown to play roles in cytokinesis, extracellular vesicle formation and viral egress. Remarkably, metagenome assemblies of Asgard archaea, the closest known living relatives of eukaryotes, were recently shown to encode homologues of the entire cascade involved in ubiquitin-mediated membrane remodelling, including ubiquitin itself, components of the ESCRT-I and ESCRT-II subcomplexes, and ESCRT-III and Vps4. Here, we explore the phylogeny, structure, and biochemistry of Asgard homologues of the ESCRT machinery and the associated ubiquitylation system. We provide evidence for the ESCRT-I and ESCRT-II subcomplexes being involved in ubiquitin-directed recruitment of ESCRT-III, as it is in eukaryotes. Taken together, our analyses suggest a pre-eukaryotic origin for the ubiquitin-coupled ESCRT system and a likely path of ESCRT evolution via a series of gene duplication and diversification events.

PubMed: 35697693
DOI: 10.1038/s41467-022-30656-2

実験手法

X-RAY DIFFRACTION (1.8 Å)