7P9M

BrxU, GmrSD-family Type IV restriction enzyme

7P9M の概要

• エントリーDOI: 10.2210/pdb7p9m/pdb
• 関連するPDBエントリー: 7P9K
• 分子名称: DUF262 domain-containing protein, SULFATE ION, CHLORIDE ION (3 entities in total)
• 機能のキーワード: restriction endonuclease, phage defence protein, dna binding protein
• 由来する生物種: Escherichia fergusonii (strain ATCC 35469 / DSM 13698 / CCUG 18766 / IAM 14443 / JCM 21226 / LMG 7866 / NBRC 102419 / NCTC 12128 / CDC 0568-73)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 136301.27

構造登録者

Picton, D.M.,Luyten, Y.,Morgan, R.D.,Nelson, A.,Smith, D.L.,Dryden, D.T.F.,Hinton, J.C.D.,Blower, T.R. (登録日: 2021-07-27, 公開日: 2021-12-08, 最終更新日: 2024-03-20)

主引用文献

Picton, D.M.,Luyten, Y.A.,Morgan, R.D.,Nelson, A.,Smith, D.L.,Dryden, D.T.F.,Hinton, J.C.D.,Blower, T.R.
The phage defence island of a multidrug resistant plasmid uses both BREX and type IV restriction for complementary protection from viruses.
Nucleic Acids Res., 49:11257-11273, 2021

PubMed Abstract: Bacteria have evolved a multitude of systems to prevent invasion by bacteriophages and other mobile genetic elements. Comparative genomics suggests that genes encoding bacterial defence mechanisms are often clustered in 'defence islands', providing a concerted level of protection against a wider range of attackers. However, there is a comparative paucity of information on functional interplay between multiple defence systems. Here, we have functionally characterised a defence island from a multidrug resistant plasmid of the emerging pathogen Escherichia fergusonii. Using a suite of thirty environmentally-isolated coliphages, we demonstrate multi-layered and robust phage protection provided by a plasmid-encoded defence island that expresses both a type I BREX system and the novel GmrSD-family type IV DNA modification-dependent restriction enzyme, BrxU. We present the structure of BrxU to 2.12 Å, the first structure of the GmrSD family of enzymes, and show that BrxU can utilise all common nucleotides and a wide selection of metals to cleave a range of modified DNAs. Additionally, BrxU undergoes a multi-step reaction cycle instigated by an unexpected ATP-dependent shift from an intertwined dimer to monomers. This direct evidence that bacterial defence islands can mediate complementary layers of phage protection enhances our understanding of the ever-expanding nature of phage-bacterial interactions.

PubMed: 34657954
DOI: 10.1093/nar/gkab906

実験手法

X-RAY DIFFRACTION (2.85 Å)