7P8V

The structure of E. coli MutL bound to a 3' resected DNA end

7P8V の概要

• エントリーDOI: 10.2210/pdb7p8v/pdb
• EMDBエントリー: 13255
• 分子名称: DNA mismatch repair protein MutL, Template strand, Primer strand, ... (5 entities in total)
• 機能のキーワード: dna mismatch repair, protein-dna complex, dna binding protein
• 由来する生物種: Escherichia coli (strain K12); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 147855.03

構造登録者

Borsellini, A.,Lamers, M.H. (登録日: 2021-07-23, 公開日: 2022-06-29, 最終更新日: 2024-07-17)

主引用文献

Borsellini, A.,Lebbink, J.H.G.,Lamers, M.H.
MutL binds to 3' resected DNA ends and blocks DNA polymerase access.
Nucleic Acids Res., 50:6224-6234, 2022

PubMed Abstract: DNA mismatch repair removes mis-incorporated bases after DNA replication and reduces the error rate a 100-1000-fold. After recognition of a mismatch, a large section of up to a thousand nucleotides is removed from the daughter strand followed by re-synthesis. How these opposite activities are coordinated is poorly understood. Here we show that the Escherichia coli MutL protein binds to the 3' end of the resected strand and blocks access of Pol I and Pol III. The cryo-EM structure of an 85-kDa MutL-DNA complex, determined to 3.7 Å resolution, reveals a unique DNA binding mode that positions MutL at the 3' end of a primer-template, but not at a 5' resected DNA end or a blunt DNA end. Hence, our work reveals a novel role for MutL in the final stages of mismatch repair by preventing premature DNA synthesis during removal of the mismatched strand.

PubMed: 35670670
DOI: 10.1093/nar/gkac432

実験手法

ELECTRON MICROSCOPY (3.6 Å)