7P8E

Crystal structure of the Receiver domain of M. truncatula cytokinin receptor MtCRE1

7P8E の概要

• エントリーDOI: 10.2210/pdb7p8e/pdb
• 分子名称: Receiver domain of histidine kinase, CALCIUM ION (3 entities in total)
• 機能のキーワード: flavodoxin-like fold, cytokinin, phosphorelay, signaling protein
• 由来する生物種: Medicago truncatula (Barrel medic, Medicago tribuloides)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 71366.50

構造登録者

Tran, L.H.,Urbanowicz, A.,Jasinski, M.,Jaskolski, M.,Ruszkowski, M. (登録日: 2021-07-21, 公開日: 2021-10-20, 最終更新日: 2024-01-31)

主引用文献

Tran, L.H.,Urbanowicz, A.,Jasinski, M.,Jaskolski, M.,Ruszkowski, M.
3D Domain Swapping Dimerization of the Receiver Domain of Cytokinin Receptor CRE1 From Arabidopsis thaliana and Medicago truncatula .
Front Plant Sci, 12:756341-756341, 2021

PubMed Abstract: Cytokinins are phytohormones regulating many biological processes that are vital to plants. CYTOKININ RESPONSE1 (CRE1), the main cytokinin receptor, has a modular architecture composed of a cytokinin-binding CHASE (Cyclases/Histidine kinases Associated Sensory Extracellular) domain, followed by a transmembrane fragment, an intracellular histidine kinase (HK) domain, and a receiver domain (REC). Perception of cytokinin signaling involves (i) a hormone molecule binding to the CHASE domain, (ii) CRE1 autophosphorylation at a conserved His residue in the HK domain, followed by a phosphorelay to (iii) a conserved Asp residue in the REC domain, (iv) a histidine-containing phosphotransfer protein (HPt), and (v) a response regulator (RR). This work focuses on the crystal structures of the REC domain of CRE1 from the model plant and from the model legume . Both REC domains form tight 3D-domain-swapped dimers. Dimerization of the REC domain agrees with the quaternary assembly of the entire CRE1 but is incompatible with a model of its complex with HPt, suggesting that a considerable conformational change should occur to enable the signal transduction. Indeed, phosphorylation of the REC domain can change the HPt-binding properties of CRE1, as shown by functional studies.

PubMed: 34630499
DOI: 10.3389/fpls.2021.756341

実験手法

X-RAY DIFFRACTION (2.5 Å)