7P4T

Tetrameric structure of murine SapA

7P4T の概要

• エントリーDOI: 10.2210/pdb7p4t/pdb
• 分子名称: Saposin-A (1 entity in total)
• 機能のキーワード: saposin, disulfide, lipid transfer, lipid binding protein
• 由来する生物種: Mus musculus (Mouse)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 36493.58

構造登録者

Shamin, M.,Deane, J.E. (登録日: 2021-07-13, 公開日: 2022-05-25, 最終更新日: 2024-10-16)

主引用文献

Shamin, M.,Spratley, S.J.,Graham, S.C.,Deane, J.E.
A Tetrameric Assembly of Saposin A: Increasing Structural Diversity in Lipid Transfer Proteins.
Contact, 4:251525642110523-251525642110523, 2021

PubMed Abstract: Saposins are lipid transfer proteins required for the degradation of sphingolipids in the lysosome. These small proteins bind lipids by transitioning from a closed, monomeric state to an open conformation exposing a hydrophobic surface that binds and shields hydrophobic lipid tails from the aqueous environment. Saposins form a range of multimeric assemblies to encompass these bound lipids and present them to hydrolases in the lysosome. This lipid-binding property of human saposin A has been exploited to form lipoprotein nanodiscs suitable for structural studies of membrane proteins. Here we present the crystal structure of a unique tetrameric assembly of murine saposin A produced serendipitously, following modifications of published protocols for making lipoprotein nanodiscs. The structure of this new saposin oligomer highlights the diversity of tertiary arrangement that can be adopted by these important lipid transfer proteins.

PubMed: 37143956
DOI: 10.1177/25152564211052382

実験手法

X-RAY DIFFRACTION (3.17 Å)