7P3A

N-terminal domain of CGI-99

7P3A の概要

• エントリーDOI: 10.2210/pdb7p3a/pdb
• 分子名称: RNA transcription, translation and transport factor protein, ISOPROPYL ALCOHOL, (4S)-2-METHYL-2,4-PENTANEDIOL, ... (5 entities in total)
• 機能のキーワード: calponin homology domain, hcle, rtraf, c14orf166, unknown function
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 25679.89

構造登録者

Kroupova, A.,Jinek, M. (登録日: 2021-07-07, 公開日: 2021-12-22, 最終更新日: 2024-10-16)

主引用文献

Kroupova, A.,Ackle, F.,Asanovic, I.,Weitzer, S.,Boneberg, F.M.,Faini, M.,Leitner, A.,Chui, A.,Aebersold, R.,Martinez, J.,Jinek, M.
Molecular architecture of the human tRNA ligase complex.
Elife, 10:-, 2021

PubMed Abstract: RtcB enzymes are RNA ligases that play essential roles in tRNA splicing, unfolded protein response, and RNA repair. In metazoa, RtcB functions as part of a five-subunit tRNA ligase complex (tRNA-LC) along with Ddx1, Cgi-99, Fam98B, and Ashwin. The human tRNA-LC or its individual subunits have been implicated in additional cellular processes including microRNA maturation, viral replication, DNA double-strand break repair, and mRNA transport. Here, we present a biochemical analysis of the inter-subunit interactions within the human tRNA-LC along with crystal structures of the catalytic subunit RTCB and the N-terminal domain of CGI-99. We show that the core of the human tRNA-LC is assembled from RTCB and the C-terminal alpha-helical regions of DDX1, CGI-99, and FAM98B, all of which are required for complex integrity. The N-terminal domain of CGI-99 displays structural homology to calponin-homology domains, and CGI-99 and FAM98B associate via their N-terminal domains to form a stable subcomplex. The crystal structure of GMP-bound RTCB reveals divalent metal coordination geometry in the active site, providing insights into its catalytic mechanism. Collectively, these findings shed light on the molecular architecture and mechanism of the human tRNA ligase complex and provide a structural framework for understanding its functions in cellular RNA metabolism.

PubMed: 34854379
DOI: 10.7554/eLife.71656

実験手法

X-RAY DIFFRACTION (2 Å)