7OYT

E.coli's putrescine receptor variant PotF/D (4JDF) with mutations E39D F88L in complex with spermidine

7OYT の概要

• エントリーDOI: 10.2210/pdb7oyt/pdb
• 分子名称: Putrescine-binding periplasmic protein PotF, SPERMIDINE, GLYCEROL, ... (9 entities in total)
• 機能のキーワード: e.coli, periplasmic binding protein, potf, spermidine, transport protein
• 由来する生物種: Escherichia coli (strain K12)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 41338.15

構造登録者

Shanmugaratnam, S.,Kroeger, P.,Hocker, B. (登録日: 2021-06-25, 公開日: 2021-12-01, 最終更新日: 2024-10-16)

主引用文献

Kroger, P.,Shanmugaratnam, S.,Scheib, U.,Hocker, B.
Fine-tuning spermidine binding modes in the putrescine binding protein PotF.
J.Biol.Chem., 297:101419-101419, 2021

PubMed Abstract: A profound understanding of the molecular interactions between receptors and ligands is important throughout diverse research, such as protein design, drug discovery, or neuroscience. What determines specificity and how do proteins discriminate against similar ligands? In this study, we analyzed factors that determine binding in two homologs belonging to the well-known superfamily of periplasmic binding proteins, PotF and PotD. Building on a previously designed construct, modes of polyamine binding were swapped. This change of specificity was approached by analyzing local differences in the binding pocket as well as overall conformational changes in the protein. Throughout the study, protein variants were generated and characterized structurally and thermodynamically, leading to a specificity swap and improvement in affinity. This dataset not only enriches our knowledge applicable to rational protein design but also our results can further lay groundwork for engineering of specific biosensors as well as help to explain the adaptability of pathogenic bacteria.

PubMed: 34801550
DOI: 10.1016/j.jbc.2021.101419

実験手法

X-RAY DIFFRACTION (1.6 Å)