7OVW

Binding domain of botulinum neurotoxin E in complex with GD1a

これはPDB形式変換不可エントリーです。

7OVW の概要

• エントリーDOI: 10.2210/pdb7ovw/pdb
• 分子名称: Neurotoxin type E, N-acetyl-alpha-neuraminic acid-(2-3)-beta-D-galactopyranose-(1-3)-2-acetamido-2-deoxy-beta-D-galactopyranose-(1-4)-beta-D-galactopyranose-(1-4)-beta-D-glucopyranose, N-acetyl-alpha-neuraminic acid-(2-3)-beta-D-galactopyranose-(1-3)-2-acetamido-2-deoxy-beta-D-galactopyranose-(1-4)-beta-D-galactopyranose, ... (4 entities in total)
• 機能のキーワード: botulinum neurotoxin, ganglioside, toxin receptor, bacterial toxin, carbohydrate binding, toxin
• 由来する生物種: Clostridium botulinum
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 110494.68

構造登録者

Masuyer, G.,Stenmark, P. (登録日: 2021-06-15, 公開日: 2021-08-11, 最終更新日: 2024-01-31)

主引用文献

Masuyer, G.,Davies, J.R.,Stenmark, P.
Mechanism of Ganglioside Receptor Recognition by Botulinum Neurotoxin Serotype E.
Int J Mol Sci, 22:-, 2021

PubMed Abstract: The botulinum neurotoxins are potent molecules that are not only responsible for the lethal paralytic disease botulism, but have also been harnessed for therapeutic uses in the treatment of an increasing number of chronic neurological and neuromuscular disorders, in addition to cosmetic applications. The toxins act at the cholinergic nerve terminals thanks to an efficient and specific mechanism of cell recognition which is based on a dual receptor system that involves gangliosides and protein receptors. Binding to surface-anchored gangliosides is the first essential step in this process. Here, we determined the X-ray crystal structure of the binding domain of BoNT/E, a toxin of clinical interest, in complex with its GD1a oligosaccharide receptor. Beyond confirmation of the conserved ganglioside binding site, we identified key interacting residues that are unique to BoNT/E and a significant rearrangement of loop 1228-1237 upon carbohydrate binding. These observations were also supported by thermodynamic measurements of the binding reaction and assessment of ganglioside selectivity by immobilised-receptor binding assays. These results provide a structural basis to understand the specificity of BoNT/E for complex gangliosides.

PubMed: 34361086
DOI: 10.3390/ijms22158315

実験手法

X-RAY DIFFRACTION (2.2 Å)