7OVR

Mature HIV-1 matrix structure

7OVR の概要

• エントリーDOI: 10.2210/pdb7ovr/pdb
• EMDBエントリー: 13079 13088
• 分子名称: HIV-1 matrix, MYRISTIC ACID, [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate (3 entities in total)
• 機能のキーワード: hiv-1, gag, matrix, virus
• 由来する生物種: Human immunodeficiency virus 1
• タンパク質・核酸の鎖数: 24
• 化学式量合計: 337438.08

構造登録者

Qu, K.,Ke, Z.L.,Zila, V.,Anders-Oesswein, M.,Glass, B.,Muecksch, F.,Mueller, R.,Schultz, C.,Mueller, B.,Kraeusslich, H.G.,Briggs, J.A.G. (登録日: 2021-06-15, 公開日: 2021-08-18, 最終更新日: 2024-11-13)

主引用文献

Qu, K.,Ke, Z.,Zila, V.,Anders-Osswein, M.,Glass, B.,Mucksch, F.,Muller, R.,Schultz, C.,Muller, B.,Krausslich, H.G.,Briggs, J.A.G.
Maturation of the matrix and viral membrane of HIV-1.
Science, 373:700-704, 2021

PubMed Abstract: Gag, the primary structural protein of HIV-1, is recruited to the plasma membrane for virus assembly by its matrix (MA) domain. Gag is subsequently cleaved into its component domains, causing structural maturation to repurpose the virion for cell entry. We determined the structure and arrangement of MA within immature and mature HIV-1 through cryo-electron tomography. We found that MA rearranges between two different hexameric lattices upon maturation. In mature HIV-1, a lipid extends out of the membrane to bind with a pocket in MA. Our data suggest that proteolytic maturation of HIV-1 not only assembles the viral capsid surrounding the genome but also repurposes the membrane-bound MA lattice for an entry or postentry function and results in the partial removal of up to 2500 lipids from the viral membrane.

PubMed: 34353956
DOI: 10.1126/science.abe6821

実験手法

ELECTRON MICROSCOPY (7 Å)