7ORN

La Crosse virus polymerase at replication initiation stage

7ORN の概要

• エントリーDOI: 10.2210/pdb7orn/pdb
• 関連するPDBエントリー: 7ORI
• EMDBエントリー: 13038 13043
• 分子名称: La Crosse virus polymerase, RNA (5'-R(P*AP*CP*GP*AP*GP*UP*GP*UP*CP*GP*UP*AP*CP*CP*AP*AP*G)-3'), RNA (5'-R(P*AP*CP*UP*UP*GP*GP*UP*AP*GP*UP*AP*CP*AP*CP*UP*AP*CP*U)-3'), ... (6 entities in total)
• 機能のキーワード: rna-dependent rna polymerase, viral protein
• 由来する生物種: La Crosse orthobunyavirus; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 278655.85

構造登録者

Arragain, B.,Durieux Trouilleton, Q.,Baudin, F.,Cusack, S.,Schoehn, G.,Malet, H. (登録日: 2021-06-06, 公開日: 2022-02-16, 最終更新日: 2024-07-17)

主引用文献

Arragain, B.,Durieux Trouilleton, Q.,Baudin, F.,Provaznik, J.,Azevedo, N.,Cusack, S.,Schoehn, G.,Malet, H.
Structural snapshots of La Crosse virus polymerase reveal the mechanisms underlying Peribunyaviridae replication and transcription.
Nat Commun, 13:902-902, 2022

PubMed Abstract: Segmented negative-strand RNA bunyaviruses encode a multi-functional polymerase that performs genome replication and transcription. Here, we establish conditions for in vitro activity of La Crosse virus polymerase and visualize its conformational dynamics by cryo-electron microscopy, unveiling the precise molecular mechanics underlying its essential activities. We find that replication initiation is coupled to distal duplex promoter formation, endonuclease movement, prime-and-realign loop extension and closure of the polymerase core that direct the template towards the active site. Transcription initiation depends on C-terminal region closure and endonuclease movements that prompt primer cleavage prior to primer entry in the active site. Product realignment after priming, observed in replication and transcription, is triggered by the prime-and-realign loop. Switch to elongation results in polymerase reorganization and core region opening to facilitate template-product duplex formation in the active site cavity. The uncovered detailed mechanics should be helpful for the future design of antivirals counteracting bunyaviral life threatening pathogens.

PubMed: 35173159
DOI: 10.1038/s41467-022-28428-z

実験手法

ELECTRON MICROSCOPY (2.8 Å)