7OQ4
Cryo-EM structure of the ATV RNAP Inhibitory Protein (RIP) bound to the DNA-binding channel of the host's RNA polymerase
Summary for 7OQ4
Entry DOI | 10.2210/pdb7oq4/pdb |
Related | 7OK0 |
EMDB information | 13026 |
Descriptor | DNA-directed RNA polymerase subunit A', DNA-directed RNA polymerase subunit L, DNA-directed RNA polymerase subunit N, ... (17 entities in total) |
Functional Keywords | viral inhibitor, transcription inhibition, atv, archaeal virus, archaea, viral protein |
Biological source | Acidianus two-tailed virus (ATV) More |
Total number of polymer chains | 14 |
Total formula weight | 423295.85 |
Authors | Pilotto, S.,Fouqueau, T.,Lukoyanova, N.,Sheppard, C.,Lucas-Staat, S.,Diaz-Santin, L.M.,Matelska, D.,Prangishvili, D.,Cheung, A.C.M.,Werner, F. (deposition date: 2021-06-02, release date: 2021-08-25, Last modification date: 2025-10-01) |
Primary citation | Pilotto, S.,Fouqueau, T.,Lukoyanova, N.,Sheppard, C.,Lucas-Staat, S.,Diaz-Santin, L.M.,Matelska, D.,Prangishvili, D.,Cheung, A.C.M.,Werner, F. Structural basis of RNA polymerase inhibition by viral and host factors. Nat Commun, 12:5523-5523, 2021 Cited by PubMed Abstract: RNA polymerase inhibition plays an important role in the regulation of transcription in response to environmental changes and in the virus-host relationship. Here we present the high-resolution structures of two such RNAP-inhibitor complexes that provide the structural bases underlying RNAP inhibition in archaea. The Acidianus two-tailed virus encodes the RIP factor that binds inside the DNA-binding channel of RNAP, inhibiting transcription by occlusion of binding sites for nucleic acid and the transcription initiation factor TFB. Infection with the Sulfolobus Turreted Icosahedral Virus induces the expression of the host factor TFS4, which binds in the RNAP funnel similarly to eukaryotic transcript cleavage factors. However, TFS4 allosterically induces a widening of the DNA-binding channel which disrupts trigger loop and bridge helix motifs. Importantly, the conformational changes induced by TFS4 are closely related to inactivated states of RNAP in other domains of life indicating a deep evolutionary conservation of allosteric RNAP inhibition. PubMed: 34535646DOI: 10.1038/s41467-021-25666-5 PDB entries with the same primary citation |
Experimental method | ELECTRON MICROSCOPY (3.27 Å) |
Structure validation
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