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7OMT

Crystal structure of ProMacrobody 21 with bound maltose

Summary for 7OMT
Entry DOI10.2210/pdb7omt/pdb
Related7OMM
EMDB information12990
Related PRD IDPRD_900001
DescriptorProMacrobody 21, alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose, HEXAETHYLENE GLYCOL, ... (5 entities in total)
Functional Keywordsnanobody cryo-em chaperone mbp, immune system
Biological sourcesynthetic construct
Total number of polymer chains1
Total formula weight57645.72
Authors
Botte, M.,Ni, D.,Schenck, S.,Zimmermann, I.,Chami, M.,Bocquet, N.,Egloff, P.,Bucher, D.,Trabuco, M.,Cheng, R.K.Y.,Brunner, J.D.,Seeger, M.A.,Stahlberg, H.,Hennig, M. (deposition date: 2021-05-24, release date: 2022-05-04, Last modification date: 2024-01-31)
Primary citationBotte, M.,Ni, D.,Schenck, S.,Zimmermann, I.,Chami, M.,Bocquet, N.,Egloff, P.,Bucher, D.,Trabuco, M.,Cheng, R.K.Y.,Brunner, J.D.,Seeger, M.A.,Stahlberg, H.,Hennig, M.
Cryo-EM structures of a LptDE transporter in complex with Pro-macrobodies offer insight into lipopolysaccharide translocation.
Nat Commun, 13:1826-1826, 2022
Cited by
PubMed Abstract: Lipopolysaccharides are major constituents of the extracellular leaflet in the bacterial outer membrane and form an effective physical barrier for environmental threats and for antibiotics in Gram-negative bacteria. The last step of LPS insertion via the Lpt pathway is mediated by the LptD/E protein complex. Detailed insights into the architecture of LptDE transporter complexes have been derived from X-ray crystallography. However, no structure of a laterally open LptD transporter, a transient state that occurs during LPS release, is available to date. Here, we report a cryo-EM structure of a partially opened LptDE transporter in complex with rigid chaperones derived from nanobodies, at 3.4 Å resolution. In addition, a subset of particles allows to model a structure of a laterally fully opened LptDE complex. Our work offers insights into the mechanism of LPS insertion, provides a structural framework for the development of antibiotics targeting LptD and describes a highly rigid chaperone scaffold to enable structural biology of challenging protein targets.
PubMed: 35383177
DOI: 10.1038/s41467-022-29459-2
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (2 Å)
Structure validation

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건을2024-11-06부터공개중

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