7OLD

Thermophilic eukaryotic 80S ribosome at pe/E (TI)-POST state

これはPDB形式変換不可エントリーです。

7OLD の概要

• エントリーDOI: 10.2210/pdb7old/pdb
• 関連するPDBエントリー: 7OLC
• EMDBエントリー: 12977
• 分子名称: 26S rRNA, 60S ribosomal protein L3-like protein, 60S ribosomal protein L4-like protein, ... (87 entities in total)
• 機能のキーワード: thermophilic eukaryotic ribosome, 80s, rrna modifications, nascent chain, ribosome
• 由来する生物種: Chaetomium thermophilum var. thermophilum DSM 1495; 詳細
• タンパク質・核酸の鎖数: 85
• 化学式量合計: 3362922.51

構造登録者

Kisonaite, M.,Wild, K.,Sinning, I. (登録日: 2021-05-19, 公開日: 2022-01-26, 最終更新日: 2026-03-04)

主引用文献

Kisonaite, M.,Wild, K.,Lapouge, K.,Ruppert, T.,Sinning, I.
High-resolution structures of a thermophilic eukaryotic 80S ribosome reveal atomistic details of translocation.
Nat Commun, 13:476-476, 2022

PubMed Abstract: Ribosomes are complex and highly conserved ribonucleoprotein assemblies catalyzing protein biosynthesis in every organism. Here we present high-resolution cryo-EM structures of the 80S ribosome from a thermophilic fungus in two rotational states, which due to increased 80S stability provide a number of mechanistic details of eukaryotic translation. We identify a universally conserved 'nested base-triple knot' in the 26S rRNA at the polypeptide tunnel exit with a bulged-out nucleotide that likely serves as an adaptable element for nascent chain containment and handover. We visualize the structure and dynamics of the ribosome protective factor Stm1 upon ribosomal 40S head swiveling. We describe the structural impact of a unique and essential macp Ψ 18S rRNA hyper-modification embracing the anticodon wobble-position for eukaryotic tRNA and mRNA translocation. We complete the eEF2-GTPase switch cycle describing the GDP-bound post-hydrolysis state. Taken together, our data and their integration into the structural landscape of 80S ribosomes furthers our understanding of protein biogenesis.

PubMed: 35079002
DOI: 10.1038/s41467-022-27967-9

実験手法

ELECTRON MICROSCOPY (3 Å)