7OKO

Structure of the outer-membrane core complex (outer ring) from a conjugative type IV secretion system

これはPDB形式変換不可エントリーです。

7OKO の概要

• エントリーDOI: 10.2210/pdb7oko/pdb
• EMDBエントリー: 12963
• 分子名称: Type IV conjugative transfer system lipoprotein TraV, TraB, Type-F conjugative transfer system secretin TraK (3 entities in total)
• 機能のキーワード: type iv secretion system, f plasmid, outer-membrane core complex, conjugation, membrane protein
• 由来する生物種: Salmonella enterica (Salmonella choleraesuis); 詳細
• タンパク質・核酸の鎖数: 65
• 化学式量合計: 1164938.25

構造登録者

Amin, H.,Ilangovan, A.,Costa, T.R.D. (登録日: 2021-05-18, 公開日: 2021-12-08, 最終更新日: 2024-07-10)

主引用文献

Amin, H.,Ilangovan, A.,Costa, T.R.D.
Architecture of the outer-membrane core complex from a conjugative type IV secretion system.
Nat Commun, 12:6834-6834, 2021

PubMed Abstract: Conjugation is one of the most important processes that bacteria utilize to spread antibiotic resistance genes among bacterial populations. Interbacterial DNA transfer requires a large double membrane-spanning nanomachine called the type 4 secretion system (T4SS) made up of the inner-membrane complex (IMC), the outer-membrane core complex (OMCC) and the conjugative pilus. The iconic F plasmid-encoded T4SS has been central in understanding conjugation for several decades, however atomic details of its structure are not known. Here, we report the structure of a complete conjugative OMCC encoded by the pED208 plasmid from E. coli, solved by cryo-electron microscopy at 3.3 Å resolution. This 2.1 MDa complex has a unique arrangement with two radial concentric rings, each having a different symmetry eventually contributing to remarkable differences in protein stoichiometry and flexibility in comparison to other OMCCs. Our structure suggests that F-OMCC is a highly dynamic complex, with implications for pilus extension and retraction during conjugation.

PubMed: 34824240
DOI: 10.1038/s41467-021-27178-8

実験手法

ELECTRON MICROSCOPY (3.4 Å)