7OF2

Structure of a human mitochondrial ribosome large subunit assembly intermediate in complex with GTPBP6.

これはPDB形式変換不可エントリーです。

7OF2 の概要

• エントリーDOI: 10.2210/pdb7of2/pdb
• 関連するPDBエントリー: 7OF0 7OF3 7OF4 7OF5 7OF6 7OF7
• EMDBエントリー: 12867
• 分子名称: 39S ribosomal protein L32, mitochondrial, 39S ribosomal protein L41, mitochondrial, 16S ribosomal RNA, ... (59 entities in total)
• 機能のキーワード: mitochondria, ribosome, biogenesis, gtpase, nsun4, mterf4
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 56
• 化学式量合計: 1853047.30

構造登録者

Hillen, H.S.,Lavdovskaia, E.,Nadler, F.,Hanitsch, E.,Linden, A.,Bohnsack, K.E.,Urlaub, H.,Richter-Dennerlein, R. (登録日: 2021-05-04, 公開日: 2021-06-02, 最終更新日: 2024-07-10)

主引用文献

Hillen, H.S.,Lavdovskaia, E.,Nadler, F.,Hanitsch, E.,Linden, A.,Bohnsack, K.E.,Urlaub, H.,Richter-Dennerlein, R.
Structural basis of GTPase-mediated mitochondrial ribosome biogenesis and recycling.
Nat Commun, 12:3672-3672, 2021

PubMed Abstract: Ribosome biogenesis requires auxiliary factors to promote folding and assembly of ribosomal proteins and RNA. Particularly, maturation of the peptidyl transferase center (PTC) is mediated by conserved GTPases, but the molecular basis is poorly understood. Here, we define the mechanism of GTPase-driven maturation of the human mitochondrial large ribosomal subunit (mtLSU) using endogenous complex purification, in vitro reconstitution and cryo-EM. Structures of transient native mtLSU assembly intermediates that accumulate in GTPBP6-deficient cells reveal how the biogenesis factors GTPBP5, MTERF4 and NSUN4 facilitate PTC folding. Addition of recombinant GTPBP6 reconstitutes late mtLSU biogenesis in vitro and shows that GTPBP6 triggers a molecular switch and progression to a near-mature PTC state. Additionally, cryo-EM analysis of GTPBP6-treated mature mitochondrial ribosomes reveals the structural basis for the dual-role of GTPBP6 in ribosome biogenesis and recycling. Together, these results provide a framework for understanding step-wise PTC folding as a critical conserved quality control checkpoint.

PubMed: 34135319
DOI: 10.1038/s41467-021-23702-y

実験手法

ELECTRON MICROSCOPY (2.7 Å)