7OCK

MAT in complex with SAMH

7OCK の概要

• エントリーDOI: 10.2210/pdb7ock/pdb
• EMDBエントリー: 12809
• 分子名称: S-adenosylmethionine synthase, SAM hydrolase (2 entities in total)
• 機能のキーワード: enzyme filamentation, metabolic regulation, phage-host interaction, cryo-em, hydrolase
• 由来する生物種: Escherichia coli (strain K12); 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 414072.06

構造登録者

Simon, H.,Kleiner, D.,Shmulevich, F.,Zarivach, R.,Zalk, R.,Tang, H.,Ding, F.,Bershtein, S. (登録日: 2021-04-27, 公開日: 2021-07-21, 最終更新日: 2025-07-02)

主引用文献

Simon-Baram, H.,Kleiner, D.,Shmulevich, F.,Zarivach, R.,Zalk, R.,Tang, H.,Ding, F.,Bershtein, S.
SAMase of Bacteriophage T3 Inactivates Escherichia coli's Methionine S -Adenosyltransferase by Forming Heteropolymers.
Mbio, 12:e0124221-e0124221, 2021

PubMed Abstract: -Adenosylmethionine lyase (SAMase) of bacteriophage T3 degrades the intracellular SAM pools of the host Escherichia coli cells, thereby inactivating a crucial metabolite involved in a plethora of cellular functions, including DNA methylation. SAMase is the first viral protein expressed upon infection, and its activity prevents methylation of the T3 genome. Maintenance of the phage genome in a fully unmethylated state has a profound effect on the infection strategy. It allows T3 to shift from a lytic infection under normal growth conditions to a transient lysogenic infection under glucose starvation. Using single-particle cryoelectron microscopy (cryo-EM) and biochemical assays, we demonstrate that SAMase performs its function by not only degrading SAM but also by interacting with and efficiently inhibiting the host's methionine -adenosyltransferase (MAT), the enzyme that produces SAM. Specifically, SAMase triggers open-ended head-to-tail assembly of E. coli MAT into an unusual linear filamentous structure in which adjacent MAT tetramers are joined by two SAMase dimers. Molecular dynamics simulations together with normal mode analyses suggest that the entrapment of MAT tetramers within filaments leads to an allosteric inhibition of MAT activity due to a shift to low-frequency, high-amplitude active-site-deforming modes. The amplification of uncorrelated motions between active-site residues weakens MAT's substrate binding affinity, providing a possible explanation for the observed loss of function. We propose that the dual function of SAMase as an enzyme that degrades SAM and as an inhibitor of MAT activity has emerged to achieve an efficient depletion of the intracellular SAM pools. Self-assembly of enzymes into filamentous structures in response to specific metabolic cues has recently emerged as a widespread strategy of metabolic regulation. In many instances, filamentation of metabolic enzymes occurs in response to starvation and leads to functional inactivation. Here, we report that bacteriophage T3 modulates the metabolism of the host E. coli cells by recruiting a similar strategy: silencing a central metabolic enzyme by subjecting it to phage-mediated polymerization. This observation points to an intriguing possibility that virus-induced polymerization of the host metabolic enzymes is a common mechanism implemented by viruses to metabolically reprogram and subdue infected cells.

PubMed: 34340545
DOI: 10.1128/mBio.01242-21

実験手法

ELECTRON MICROSCOPY (3.6 Å)