7OBJ

Carbon regulatory PII-like protein SbtB from Synechocystis sp. 6803 in complex with cyclic di-AMP (c-di-AMP)

7OBJ の概要

• エントリーDOI: 10.2210/pdb7obj/pdb
• 分子名称: Membrane-associated protein slr1513, (2R,3R,3aS,5R,7aR,9R,10R,10aS,12R,14aR)-2,9-bis(6-amino-9H-purin-9-yl)octahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8 ]tetraoxadiphosphacyclododecine-3,5,10,12-tetrol 5,12-dioxide (3 entities in total)
• 機能のキーワード: sbtb, carbon sensing, pii-like, cyanobacteria, signaling protein
• 由来する生物種: Synechocystis sp. PCC 6803
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 40983.15

構造登録者

Selim, K.A.,Albrecht, R.,Hartmann, M.D. (登録日: 2021-04-22, 公開日: 2022-02-02, 最終更新日: 2024-11-20)

主引用文献

Selim, K.A.,Haffner, M.,Burkhardt, M.,Mantovani, O.,Neumann, N.,Albrecht, R.,Seifert, R.,Kruger, L.,Stulke, J.,Hartmann, M.D.,Hagemann, M.,Forchhammer, K.
Diurnal metabolic control in cyanobacteria requires perception of second messenger signaling molecule c-di-AMP by the carbon control protein SbtB.
Sci Adv, 7:eabk0568-eabk0568, 2021

PubMed Abstract: Because of their photosynthesis-dependent lifestyle, cyanobacteria evolved sophisticated regulatory mechanisms to adapt to oscillating day-night metabolic changes. How they coordinate the metabolic switch between autotrophic and glycogen-catabolic metabolism in light and darkness is poorly understood. Recently, c-di-AMP has been implicated in diurnal regulation, but its mode of action remains elusive. To unravel the signaling functions of c-di-AMP in cyanobacteria, we isolated c-di-AMP receptor proteins. Thereby, the carbon-sensor protein SbtB was identified as a major c-di-AMP receptor, which we confirmed biochemically and by x-ray crystallography. In search for the c-di-AMP signaling function of SbtB, we found that both SbtB and c-di-AMP cyclase–deficient mutants showed reduced diurnal growth and that c-di-AMP–bound SbtB interacts specifically with the glycogen-branching enzyme GlgB. Accordingly, both mutants displayed impaired glycogen synthesis during the day and impaired nighttime survival. Thus, the pivotal role of c-di-AMP in day-night acclimation can be attributed to SbtB-mediated regulation of glycogen metabolism.

PubMed: 34878830
DOI: 10.1126/sciadv.abk0568

実験手法

X-RAY DIFFRACTION (2 Å)