7OA8

PilC minor pilin of Streptococcus sanguinis SK36 type IV pili

7OA8 の概要

• エントリーDOI: 10.2210/pdb7oa8/pdb
• 分子名称: PilC minor pilin (2 entities in total)
• 機能のキーワード: pilin, structural protein
• 由来する生物種: Streptococcus sanguinis
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 51515.57

構造登録者

Sheppard, D.,Pelicic, V. (登録日: 2021-04-19, 公開日: 2022-05-04, 最終更新日: 2024-01-31)

主引用文献

Shahin, M.,Sheppard, D.,Raynaud, C.,Berry, J.L.,Gurung, I.,Silva, L.M.,Feizi, T.,Liu, Y.,Pelicic, V.
Characterization of a glycan-binding complex of minor pilins completes the analysis of Streptococcus sanguinis type 4 pili subunits.
Proc.Natl.Acad.Sci.USA, 120:e2216237120-e2216237120, 2023

PubMed Abstract: Type 4 filaments (T4F)-of which type 4 pili (T4P) are the archetype-are a superfamily of nanomachines nearly ubiquitous in prokaryotes. T4F are polymers of one major pilin, which also contain minor pilins whose roles are often poorly understood. Here, we complete the structure/function analysis of the full set of T4P pilins in the opportunistic bacterial pathogen . We determined the structure of the minor pilin PilA, which is unexpectedly similar to one of the subunits of a tip-located complex of four minor pilins, widely conserved in T4F. We found that PilA interacts and dramatically stabilizes the minor pilin PilC. We determined the structure of PilC, showing that it is a modular pilin with a lectin module binding a subset of glycans prevalent in the human glycome, the host of . Altogether, our findings support a model whereby the minor pilins in T4P form a tip-located complex promoting adhesion to various host receptors. This has general implications for T4F.

PubMed: 36626560
DOI: 10.1073/pnas.2216237120

実験手法

X-RAY DIFFRACTION (1.6 Å)