7O9K

Human mitochondrial ribosome large subunit assembly intermediate with MTERF4-NSUN4, MRM2, MTG1, the MALSU module, GTPBP5 and mtEF-Tu

これはPDB形式変換不可エントリーです。

7O9K の概要

• エントリーDOI: 10.2210/pdb7o9k/pdb
• EMDBエントリー: 12763
• 分子名称: 39S ribosomal protein L32, mitochondrial, 39S ribosomal protein L40, mitochondrial, 39S ribosomal protein L41, mitochondrial, ... (70 entities in total)
• 機能のキーワード: mitochondria, gtpase, ribosome assembly intermediate, ribosome
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 69
• 化学式量合計: 2196275.88

構造登録者

Valentin Gese, G.,Hallberg, B.M. (登録日: 2021-04-16, 公開日: 2021-06-30, 最終更新日: 2025-12-10)

主引用文献

Cipullo, M.,Gese, G.V.,Khawaja, A.,Hallberg, B.M.,Rorbach, J.
Structural basis for late maturation steps of the human mitoribosomal large subunit.
Nat Commun, 12:3673-3673, 2021

PubMed Abstract: Mitochondrial ribosomes (mitoribosomes) synthesize a critical set of proteins essential for oxidative phosphorylation. Therefore, mitoribosomal function is vital to the cellular energy supply. Mitoribosome biogenesis follows distinct molecular pathways that remain poorly understood. Here, we determine the cryo-EM structures of mitoribosomes isolated from human cell lines with either depleted or overexpressed mitoribosome assembly factor GTPBP5, allowing us to capture consecutive steps during mitoribosomal large subunit (mt-LSU) biogenesis. Our structures provide essential insights into the last steps of 16S rRNA folding, methylation and peptidyl transferase centre (PTC) completion, which require the coordinated action of nine assembly factors. We show that mammalian-specific MTERF4 contributes to the folding of 16S rRNA, allowing 16 S rRNA methylation by MRM2, while GTPBP5 and NSUN4 promote fine-tuning rRNA rearrangements leading to PTC formation. Moreover, our data reveal an unexpected involvement of the elongation factor mtEF-Tu in mt-LSU assembly, where mtEF-Tu interacts with GTPBP5, similar to its interaction with tRNA during translational elongation.

PubMed: 34135318
DOI: 10.1038/s41467-021-23617-8

実験手法

ELECTRON MICROSCOPY (3.1 Å)