7O6R

Crystal structure of bovine Hsc70(aa1-554)E213A/D214A in complex with 1H-Indazole

Summary for 7O6R

• Entry DOI: 10.2210/pdb7o6r/pdb
• Related: 7ODB 7ODD 7ODI
• Descriptor: Heat shock cognate 71 kDa protein, 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID, 1H-indazole, ... (8 entities in total)
• Functional Keywords: chaperone
• Biological source: Bos taurus (Bovine)
• Total number of polymer chains: 1
• Total formula weight: 61658.84

Authors

Zehe, M.,Grimm, C.,Sotriffer, C. (deposition date: 2021-04-12, release date: 2022-05-04, Last modification date: 2024-02-14)

Primary citation

Zehe, M.,Kehrein, J.,Schollmayer, C.,Plank, C.,Kovacs, H.,Merino Asumendi, E.,Holzgrabe, U.,Grimm, C.,Sotriffer, C.
Combined In-Solution Fragment Screening and Crystallographic Binding-Mode Analysis with a Two-Domain Hsp70 Construct.
Acs Chem.Biol., 2024

PubMed Abstract: Heat shock protein 70 (Hsp70) isoforms are key players in the regulation of protein homeostasis and cell death pathways and are therefore attractive targets in cancer research. Developing nucleotide-competitive inhibitors or allosteric modulators, however, has turned out to be very challenging for this protein family, and no Hsp70-directed therapeutics have so far become available. As the field could profit from alternative starting points for inhibitor development, we present the results of a fragment-based screening approach on a two-domain Hsp70 construct using in-solution NMR methods, together with X-ray-crystallographic investigations and mixed-solvent molecular dynamics simulations. The screening protocol resulted in hits on both domains. In particular, fragment binding in a deeply buried pocket at the substrate-binding domain could be detected. The corresponding site is known to be important for communication between the nucleotide-binding and substrate-binding domains of Hsp70 proteins. The main fragment identified at this position also offers an interesting starting point for the development of a dual Hsp70/Hsp90 inhibitor.

PubMed: 38317495
DOI: 10.1021/acschembio.3c00589

Experimental method

X-RAY DIFFRACTION (2 Å)