7O4L

Yeast TFIIH in the expanded state within the pre-initiation complex

7O4L の概要

• エントリーDOI: 10.2210/pdb7o4l/pdb
• EMDBエントリー: 12722
• 分子名称: General transcription and DNA repair factor IIH helicase subunit XPD, DNA-directed RNA polymerase II subunit RPB2, DNA-directed RNA polymerase II subunit RPB4, ... (22 entities in total)
• 機能のキーワード: pre-initiation complex, transcription
• 由来する生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast); 詳細
• タンパク質・核酸の鎖数: 17
• 化学式量合計: 1008868.11

構造登録者

Schilbach, S.,Aibara, S.,Dienemann, C.,Grabbe, F.,Cramer, P. (登録日: 2021-04-06, 公開日: 2021-06-16, 最終更新日: 2024-07-10)

主引用文献

Schilbach, S.,Aibara, S.,Dienemann, C.,Grabbe, F.,Cramer, P.
Structure of RNA polymerase II pre-initiation complex at 2.9 angstrom defines initial DNA opening.
Cell, 184:4064-4072.e28, 2021

PubMed Abstract: Transcription initiation requires assembly of the RNA polymerase II (Pol II) pre-initiation complex (PIC) and opening of promoter DNA. Here, we present the long-sought high-resolution structure of the yeast PIC and define the mechanism of initial DNA opening. We trap the PIC in an intermediate state that contains half a turn of open DNA located 30-35 base pairs downstream of the TATA box. The initially opened DNA region is flanked and stabilized by the polymerase "clamp head loop" and the TFIIF "charged region" that both contribute to promoter-initiated transcription. TFIIE facilitates initiation by buttressing the clamp head loop and by regulating the TFIIH translocase. The initial DNA bubble is then extended in the upstream direction, leading to the open promoter complex and enabling start-site scanning and RNA synthesis. This unique mechanism of DNA opening may permit more intricate regulation than in the Pol I and Pol III systems.

PubMed: 34133942
DOI: 10.1016/j.cell.2021.05.012

実験手法

ELECTRON MICROSCOPY (3.4 Å)