7O21

Structure of Bdellovibrio bacteriovorus Bd1075

7O21 の概要

• エントリーDOI: 10.2210/pdb7o21/pdb
• 分子名称: Uncharacterized conserved, BROMIDE ION, THIOCYANATE ION, ... (6 entities in total)
• 機能のキーワード: peptidoglycan ld-carboxypeptidase ntf2, hydrolase
• 由来する生物種: Bdellovibrio bacteriovorus (strain ATCC 15356 / DSM 50701 / NCIB 9529 / HD100)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 76787.37

構造登録者

Lovering, A.L.,Valdivia-Delgado, M. (登録日: 2021-03-30, 公開日: 2021-04-21, 最終更新日: 2024-06-19)

主引用文献

Banks, E.J.,Valdivia-Delgado, M.,Biboy, J.,Wilson, A.,Cadby, I.T.,Vollmer, W.,Lambert, C.,Lovering, A.L.,Sockett, R.E.
Asymmetric peptidoglycan editing generates cell curvature in Bdellovibrio predatory bacteria.
Nat Commun, 13:1509-1509, 2022

PubMed Abstract: Peptidoglycan hydrolases contribute to the generation of helical cell shape in Campylobacter and Helicobacter bacteria, while cytoskeletal or periskeletal proteins determine the curved, vibrioid cell shape of Caulobacter and Vibrio. Here, we identify a peptidoglycan hydrolase in the vibrioid-shaped predatory bacterium Bdellovibrio bacteriovorus which invades and replicates within the periplasm of Gram-negative prey bacteria. The protein, Bd1075, generates cell curvature in B. bacteriovorus by exerting LD-carboxypeptidase activity upon the predator cell wall as it grows inside spherical prey. Bd1075 localizes to the outer convex face of B. bacteriovorus; this asymmetric localization requires a nuclear transport factor 2-like (NTF2) domain at the protein C-terminus. We solve the crystal structure of Bd1075, which is monomeric with key differences to other LD-carboxypeptidases. Rod-shaped Δbd1075 mutants invade prey more slowly than curved wild-type predators and stretch invaded prey from within. We therefore propose that the vibrioid shape of B. bacteriovorus contributes to predatory fitness.

PubMed: 35314810
DOI: 10.1038/s41467-022-29007-y

実験手法

X-RAY DIFFRACTION (1.34 Å)