7NZC

First SH3 domain of POSH (Plenty of SH3 Domains protein)

これはPDB形式変換不可エントリーです。

7NZC の概要

• エントリーDOI: 10.2210/pdb7nzc/pdb
• 分子名称: E3 ubiquitin-protein ligase SH3RF1, 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID (3 entities in total)
• 機能のキーワード: map-kinase scaffold activity ubiquitin protein ligase activity, signaling protein
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 7537.66

構造登録者

Palencia, A.,Bessa, L.M.,Jensen, M.R. (登録日: 2021-03-23, 公開日: 2021-12-22, 最終更新日: 2024-01-31)

主引用文献

Marino Perez, L.,Ielasi, F.S.,Bessa, L.M.,Maurin, D.,Kragelj, J.,Blackledge, M.,Salvi, N.,Bouvignies, G.,Palencia, A.,Jensen, M.R.
Visualizing protein breathing motions associated with aromatic ring flipping.
Nature, 602:695-700, 2022

PubMed Abstract: Aromatic residues cluster in the core of folded proteins, where they stabilize the structure through multiple interactions. Nuclear magnetic resonance (NMR) studies in the 1970s showed that aromatic side chains can undergo ring flips-that is, 180° rotations-despite their role in maintaining the protein fold. It was suggested that large-scale 'breathing' motions of the surrounding protein environment would be necessary to accommodate these ring flipping events. However, the structural details of these motions have remained unclear. Here we uncover the structural rearrangements that accompany ring flipping of a buried tyrosine residue in an SH3 domain. Using NMR, we show that the tyrosine side chain flips to a low-populated, minor state and, through a proteome-wide sequence analysis, we design mutants that stabilize this state, which allows us to capture its high-resolution structure by X-ray crystallography. A void volume is generated around the tyrosine ring during the structural transition between the major and minor state, and this allows fast flipping to take place. Our results provide structural insights into the protein breathing motions that are associated with ring flipping. More generally, our study has implications for protein design and structure prediction by showing how the local protein environment influences amino acid side chain conformations and vice versa.

PubMed: 35173330
DOI: 10.1038/s41586-022-04417-6

実験手法

X-RAY DIFFRACTION (1.111 Å)