7NWJ

Solution NMR structure of the N-terminal domain of CEP164 (1-109)

7NWJ の概要

• エントリーDOI: 10.2210/pdb7nwj/pdb
• NMR情報: BMRB: 50793
• 分子名称: Centrosomal protein of 164 kDa (1 entity in total)
• 機能のキーワード: centriolar protein, ttbk2 binding, ciliopathies, protein binding
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 12950.51

構造登録者

van Breugel, M.,Rutherford, T.J. (登録日: 2021-03-16, 公開日: 2021-09-15, 最終更新日: 2024-06-19)

主引用文献

Rosa E Silva, I.,Bino, L.,Johnson, C.M.,Rutherford, T.J.,Neuhaus, D.,Andreeva, A.,Cajanek, L.,van Breugel, M.
Molecular mechanisms underlying the role of the centriolar CEP164-TTBK2 complex in ciliopathies.
Structure, 30:114-128.e9, 2022

PubMed Abstract: Cilia formation is essential for human life. One of the earliest events in the ciliogenesis program is the recruitment of tau-tubulin kinase 2 (TTBK2) by the centriole distal appendage component CEP164. Due to the lack of high-resolution structural information on this complex, it is unclear how it is affected in human ciliopathies such as nephronophthisis. Furthermore, it is poorly understood if binding to CEP164 influences TTBK2 activities. Here, we present a detailed biochemical, structural, and functional analysis of the CEP164-TTBK2 complex and demonstrate how it is compromised by two ciliopathic mutations in CEP164. Moreover, we also provide insights into how binding to CEP164 is coordinated with TTBK2 activities. Together, our data deepen our understanding of a crucial step in cilia formation and will inform future studies aimed at restoring CEP164 functionality in a debilitating human ciliopathy.

PubMed: 34499853
DOI: 10.1016/j.str.2021.08.007

実験手法

SOLUTION NMR