7NUR

Structure of the Toxoplasma gondii kinase Ron13, kinase-dead mutant

7NUR の概要

• エントリーDOI: 10.2210/pdb7nur/pdb
• EMDBエントリー: 12600
• 分子名称: Protein kinase domain-containing protein (1 entity in total)
• 機能のキーワード: secretory pathway, kinase, transferase
• 由来する生物種: Toxoplasma gondii (strain ATCC 50853 / GT1)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 126419.68

構造登録者

Korkhov, V.M.,Mehta, V. (登録日: 2021-03-13, 公開日: 2021-05-26, 最終更新日: 2024-10-16)

主引用文献

Lentini, G.,Ben Chaabene, R.,Vadas, O.,Ramakrishnan, C.,Mukherjee, B.,Mehta, V.,Lunghi, M.,Grossmann, J.,Maco, B.,Visentin, R.,Hehl, A.B.,Korkhov, V.M.,Soldati-Favre, D.
Structural insights into an atypical secretory pathway kinase crucial for Toxoplasma gondii invasion.
Nat Commun, 12:3788-3788, 2021

PubMed Abstract: Active host cell invasion by the obligate intracellular apicomplexan parasites relies on the formation of a moving junction, which connects parasite and host cell plasma membranes during entry. Invading Toxoplasma gondii tachyzoites secrete their rhoptry content and insert a complex of RON proteins on the cytoplasmic side of the host cell membrane providing an anchor to which the parasite tethers. Here we show that a rhoptry-resident kinase RON13 is a key virulence factor that plays a crucial role in host cell entry. Cryo-EM, kinase assays, phosphoproteomics and cellular analyses reveal that RON13 is a secretory pathway kinase of atypical structure that phosphorylates rhoptry proteins including the components of the RON complex. Ultimately, RON13 kinase activity controls host cell invasion by anchoring the moving junction at the parasite-host cell interface.

PubMed: 34145271
DOI: 10.1038/s41467-021-24083-y

実験手法

ELECTRON MICROSCOPY (3.125 Å)