7NT8

Influenza virus H3N2 nucleoprotein - R416A mutant

7NT8 の概要

• エントリーDOI: 10.2210/pdb7nt8/pdb
• 分子名称: Glutathione S-transferase class-mu 26 kDa isozyme,Nucleoprotein (2 entities in total)
• 機能のキーワード: influenza virus, nucleoprotein, rna-binding protein, rna binding protein
• 由来する生物種: Schistosoma japonicum (Blood fluke); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 165777.66

構造登録者

Keown, J.R.,Knight, M.L.,Grimes, J.M.,Fodor, E. (登録日: 2021-03-09, 公開日: 2021-07-07, 最終更新日: 2024-01-31)

主引用文献

Knight, M.L.,Fan, H.,Bauer, D.L.V.,Grimes, J.M.,Fodor, E.,Keown, J.R.
Structure of an H3N2 influenza virus nucleoprotein.
Acta Crystallogr.,Sect.F, 77:208-214, 2021

PubMed Abstract: Influenza A viruses of the H1N1 and H3N2 subtypes are responsible for seasonal epidemic events. The influenza nucleoprotein (NP) binds to the viral genomic RNA and is essential for its replication. Efforts are under way to produce therapeutics and vaccines targeting the NP. Despite this, no structure of an NP from an H3N2 virus has previously been determined. Here, the structure of the A/Northern Territory/60/1968 (H3N2) influenza virus NP is presented at 2.2 Å resolution. The structure is highly similar to those of the A/WSN/1933 (H1N1) and A/Hong Kong/483/97 (H5N1) NPs. Nonconserved amino acids are widely dispersed both at the sequence and structural levels. A movement of the 73-90 RNA-binding loop is observed to be the key difference between the structure determined here and previous structures. The data presented here increase the understanding of structural conservation amongst influenza NPs and may aid in the design of universal interventions against influenza.

PubMed: 34196611
DOI: 10.1107/S2053230X2100635X

実験手法

X-RAY DIFFRACTION (2.22 Å)