7NSH

39S mammalian mitochondrial large ribosomal subunit with mtRRF (post) and mtEFG2

これはPDB形式変換不可エントリーです。

7NSH の概要

• エントリーDOI: 10.2210/pdb7nsh/pdb
• EMDBエントリー: 12567
• 分子名称: Mitochondrial ribosomal protein L27, Mitochondrial ribosomal protein L43, mL44, ... (61 entities in total)
• 機能のキーワード: ribosome, mitochondria, translation
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 61
• 化学式量合計: 2021114.02

構造登録者

Kummer, E.,Schubert, K.,Ban, N. (登録日: 2021-03-07, 公開日: 2021-05-05, 最終更新日: 2024-07-10)

主引用文献

Kummer, E.,Schubert, K.N.,Schoenhut, T.,Scaiola, A.,Ban, N.
Structural basis of translation termination, rescue, and recycling in mammalian mitochondria.
Mol.Cell, 81:2566-2582.e6, 2021

PubMed Abstract: The mitochondrial translation system originates from a bacterial ancestor but has substantially diverged in the course of evolution. Here, we use single-particle cryo-electron microscopy (cryo-EM) as a screening tool to identify mitochondrial translation termination mechanisms and to describe them in molecular detail. We show how mitochondrial release factor 1a releases the nascent chain from the ribosome when it encounters the canonical stop codons UAA and UAG. Furthermore, we define how the peptidyl-tRNA hydrolase ICT1 acts as a rescue factor on mitoribosomes that have stalled on truncated messages to recover them for protein synthesis. Finally, we present structural models detailing the process of mitochondrial ribosome recycling to explain how a dedicated elongation factor, mitochondrial EFG2 (mtEFG2), has specialized for cooperation with the mitochondrial ribosome recycling factor to dissociate the mitoribosomal subunits at the end of the translation process.

PubMed: 33878294
DOI: 10.1016/j.molcel.2021.03.042

実験手法

ELECTRON MICROSCOPY (3.2 Å)