7NS2

Virion of Leishmania RNA virus 1

7NS2 の概要

• エントリーDOI: 10.2210/pdb7ns2/pdb
• 関連するPDBエントリー: 6y83
• EMDBエントリー: 10722 12558
• 分子名称: Capsid protein (1 entity in total)
• 機能のキーワード: leishmania guyanensis, native virion structure, mucocutaneous leishmaniasis, virus, totiviridae
• 由来する生物種: Leishmania RNA virus 1 - LgM5313
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 172863.48

構造登録者

Prochazkova, M.,Grybchuk, D.,Fuzik, T. (登録日: 2021-03-05, 公開日: 2022-09-21, 最終更新日: 2024-10-23)

主引用文献

Prochazkova, M.,Fuzik, T.,Grybchuk, D.,Yurchenko, V.,Plevka, P.
Virion structure of Leishmania RNA virus 1.
Virology, 577:149-154, 2022

PubMed Abstract: The presence of Leishmania RNA virus 1 (LRV1) enables Leishmania protozoan parasites to cause more severe disease than the virus-free strains. The structure of LRV1 virus-like particles has been determined previously, however, the structure of the LRV1 virion has not been characterized. Here we used cryo-electron microscopy and single-particle reconstruction to determine the structures of the LRV1 virion and empty particle isolated from Leishmania guyanensis to resolutions of 4.0 Å and 3.6 Å, respectively. The capsid of LRV1 is built from sixty dimers of capsid proteins organized with icosahedral symmetry. RNA genomes of totiviruses are replicated inside the virions by RNA polymerases expressed as C-terminal extensions of a sub-population of capsid proteins. Most of the virions probably contain one or two copies of the RNA polymerase, however, the location of the polymerase domains in LRV1 capsid could not be identified, indicating that it varies among particles. Importance. Every year over 200 000 people contract leishmaniasis and more than five hundred people die of the disease. The mucocutaneous form of leishmaniasis produces lesions that can destroy the mucous membranes of the nose, mouth, and throat. Leishmania parasites carrying Leishmania RNA virus 1 (LRV1) are predisposed to cause aggravated symptoms in the mucocutaneous form of leishmaniasis. Here, we present the structure of the LRV1 virion determined using cryo-electron microscopy.

PubMed: 36371873
DOI: 10.1016/j.virol.2022.09.014

実験手法

ELECTRON MICROSCOPY (3.63 Å)