7NPA

Crystal structure of the Coenzyme F420-dependent sulfite reductase from Methanothermococcus thermolithotrophicus at 1.55-A resolution

7NPA の概要

• エントリーDOI: 10.2210/pdb7npa/pdb
• 分子名称: Coenzyme F420-dependent sulfite reductase, LITHIUM ION, DI(HYDROXYETHYL)ETHER, ... (13 entities in total)
• 機能のキーワード: sulfite detoxification, sulfur metabolism, sulfide, hydrogenotrophic methanogens, iron-sulfur cluster, sulfite-reductase evolution, sulfite/nitrite reductase, siroheme, flavin, oxidoreductase, ferredoxin, f420, thermophile
• 由来する生物種: Methanothermococcus thermolithotrophicus DSM 2095
• タンパク質・核酸の鎖数: 16
• 化学式量合計: 1184790.82

構造登録者

Jespersen, M.,Wagner, T. (登録日: 2021-02-26, 公開日: 2022-03-23, 最終更新日: 2024-02-07)

主引用文献

Jespersen, M.,Pierik, A.J.,Wagner, T.
Structures of the sulfite detoxifying F 420 -dependent enzyme from Methanococcales.
Nat.Chem.Biol., 2023

PubMed Abstract: Methanogenic archaea are main actors in the carbon cycle but are sensitive to reactive sulfite. Some methanogens use a sulfite detoxification system that combines an FH-oxidase with a sulfite reductase, both of which are proposed precursors of modern enzymes. Here, we present snapshots of this coupled system, named coenzyme F-dependent sulfite reductase (Group I Fsr), obtained from two marine methanogens. Fsr organizes as a homotetramer, harboring an intertwined six-[4Fe-4S] cluster relay characterized by spectroscopy. The wire, spanning 5.4 nm, electronically connects the flavin to the siroheme center. Despite a structural architecture similar to dissimilatory sulfite reductases, Fsr shows a siroheme coordination and a reaction mechanism identical to assimilatory sulfite reductases. Accordingly, the reaction of Fsr is unidirectional, reducing sulfite or nitrite with FH. Our results provide structural insights into this unique fusion, in which a primitive sulfite reductase turns a poison into an elementary block of life.

PubMed: 36658338
DOI: 10.1038/s41589-022-01232-y

実験手法

X-RAY DIFFRACTION (1.55 Å)