7N65

Complex structure of HIV superinfection Fab QA013.2 and BG505.SOSIP.664

これはPDB形式変換不可エントリーです。

7N65 の概要

• エントリーDOI: 10.2210/pdb7n65/pdb
• EMDBエントリー: 24195
• 分子名称: Envelope glycoprotein gp41, alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (12 entities in total)
• 機能のキーワード: hiv-1 envelope, superinfection antibody qa013.2, viral protein, viral protein-immune system complex, viral protein/immune system
• 由来する生物種: Human immunodeficiency virus 1; 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 338333.72

構造登録者

Mangala Prasad, V.,Shipley, M.M.,Overbaugh, J.M.,Lee, K.K. (登録日: 2021-06-07, 公開日: 2021-07-28)

主引用文献

Shipley, M.M.,Mangala Prasad, V.,Doepker, L.E.,Dingens, A.S.,Ralph, D.K.,Harkins, E.,Dhar, A.,Arenz, D.,Chohan, V.,Weight, H.,Mandaliya, K.,Bloom, J.D.,Matsen Iv, F.,Lee, K.K.,Overbaugh, J.M.
Functional development of a V3/glycan-specific broadly neutralizing antibody isolated from a case of HIV superinfection.
Elife, 10:-, 2021

PubMed Abstract: Stimulating broadly neutralizing antibodies (bnAbs) directly from germline remains a barrier for HIV vaccines. HIV superinfection elicits bnAbs more frequently than single infection, providing clues of how to elicit such responses. We used longitudinal antibody sequencing and structural studies to characterize bnAb development from a superinfection case. BnAb QA013.2 bound initial and superinfecting viral Env, despite its probable naive progenitor only recognizing the superinfecting strain, suggesting both viruses influenced this lineage. A 4.15 Å cryo-EM structure of QA013.2 bound to native-like trimer showed recognition of V3 signatures (N301/N332 and GDIR). QA013.2 relies less on CDRH3 and more on framework and CDRH1 for affinity and breadth compared to other V3/glycan-specific bnAbs. Antigenic profiling revealed that viral escape was achieved by changes in the structurally-defined epitope and by mutations in V1. These results highlight shared and novel properties of QA013.2 relative to other V3/glycan-specific bnAbs in the setting of sequential, diverse antigens.

PubMed: 34263727
DOI: 10.7554/eLife.68110

実験手法

ELECTRON MICROSCOPY (4.15 Å)