7N62

SARS-CoV-2 Spike (2P) in complex with C12C9 Fab (NTD local reconstruction)

7N62 の概要

• エントリーDOI: 10.2210/pdb7n62/pdb
• EMDBエントリー: 24192
• 分子名称: Spike glycoprotein, C12C9 Fab heavy chain, C12C9 Fab light chain, ... (4 entities in total)
• 機能のキーワード: coronavirus, antibody, epitope, viral protein, viral protein-immune system complex, viral protein/immune system
• 由来する生物種: Severe acute respiratory syndrome coronavirus 2 (2019-nCoV, SARS-CoV-2); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 190195.66

構造登録者

Windsor, I.W.,Jenni, S.,Bajic, G.,Tong, P.,Gautam, A.K.,Wesemann, D.R.,Harrison, S.C. (登録日: 2021-06-07, 公開日: 2021-08-04, 最終更新日: 2024-11-13)

主引用文献

Tong, P.,Gautam, A.,Windsor, I.,Travers, M.,Chen, Y.,Garcia, N.,Whiteman, N.B.,McKay, L.G.A.,Lelis, F.J.N.,Habibi, S.,Cai, Y.,Rennick, L.J.,Duprex, W.P.,McCarthy, K.R.,Lavine, C.L.,Zuo, T.,Lin, J.,Zuiani, A.,Feldman, J.,MacDonald, E.A.,Hauser, B.M.,Griffths, A.,Seaman, M.S.,Schmidt, A.G.,Chen, B.,Neuberg, D.,Bajic, G.,Harrison, S.C.,Wesemann, D.R.
Memory B cell repertoire for recognition of evolving SARS-CoV-2 spike.
Biorxiv, 2021

PubMed Abstract: Memory B cell reserves can generate protective antibodies against repeated SARS-CoV-2 infections, but with an unknown reach from original infection to antigenically drifted variants. We charted memory B cell receptor-encoded monoclonal antibodies (mAbs) from 19 COVID-19 convalescent subjects against SARS-CoV-2 spike (S) and found 7 major mAb competition groups against epitopes recurrently targeted across individuals. Inclusion of published and newly determined structures of mAb-S complexes identified corresponding epitopic regions. Group assignment correlated with cross-CoV-reactivity breadth, neutralization potency, and convergent antibody signatures. mAbs that competed for binding the original S isolate bound differentially to S variants, suggesting the protective importance of otherwise-redundant recognition. The results furnish a global atlas of the S-specific memory B cell repertoire and illustrate properties conferring robustness against emerging SARS-CoV-2 variants.

PubMed: 33758863
DOI: 10.1101/2021.03.10.434840

実験手法

ELECTRON MICROSCOPY (4 Å)