7N40

Crystal structure of LIN9-RbAp48-LIN37, a MuvB subcomplex

7N40 の概要

• エントリーDOI: 10.2210/pdb7n40/pdb
• 分子名称: Histone-binding protein RBBP4, Isoform 2 of Protein lin-9 homolog, Protein lin-37 homolog, ... (4 entities in total)
• 機能のキーワード: cell division, histone binding, transcription factor, dna binding protein, complex, muvb, cell cycle
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 73197.98

構造登録者

Asthana, A.,Ramanan, P.,Tripathi, S.M.,Rubin, S.M. (登録日: 2021-06-02, 公開日: 2022-02-09, 最終更新日: 2023-10-18)

主引用文献

Asthana, A.,Ramanan, P.,Hirschi, A.,Guiley, K.Z.,Wijeratne, T.U.,Shelansky, R.,Doody, M.J.,Narasimhan, H.,Boeger, H.,Tripathi, S.,Muller, G.A.,Rubin, S.M.
The MuvB complex binds and stabilizes nucleosomes downstream of the transcription start site of cell-cycle dependent genes.
Nat Commun, 13:526-526, 2022

PubMed Abstract: The chromatin architecture in promoters is thought to regulate gene expression, but it remains uncertain how most transcription factors (TFs) impact nucleosome position. The MuvB TF complex regulates cell-cycle dependent gene-expression and is critical for differentiation and proliferation during development and cancer. MuvB can both positively and negatively regulate expression, but the structure of MuvB and its biochemical function are poorly understood. Here we determine the overall architecture of MuvB assembly and the crystal structure of a subcomplex critical for MuvB function in gene repression. We find that the MuvB subunits LIN9 and LIN37 function as scaffolding proteins that arrange the other subunits LIN52, LIN54 and RBAP48 for TF, DNA, and histone binding, respectively. Biochemical and structural data demonstrate that MuvB binds nucleosomes through an interface that is distinct from LIN54-DNA consensus site recognition and that MuvB increases nucleosome occupancy in a reconstituted promoter. We find in arrested cells that MuvB primarily associates with a tightly positioned +1 nucleosome near the transcription start site (TSS) of MuvB-regulated genes. These results support a model that MuvB binds and stabilizes nucleosomes just downstream of the TSS on its target promoters to repress gene expression.

PubMed: 35082292
DOI: 10.1038/s41467-022-28094-1

実験手法

X-RAY DIFFRACTION (2.55 Å)