7MSU

Crystal structure of an archaeal CNNM, MtCorB, CBS-pair domain in complex with Mg2+-ATP

「7LJ6」から置き換えられました

7MSU の概要

• エントリーDOI: 10.2210/pdb7msu/pdb
• 関連するPDBエントリー: 7M1T 7M1U
• 分子名称: Hemolysin, contains CBS domains, 1,2-ETHANEDIOL, MAGNESIUM ION, ... (5 entities in total)
• 機能のキーワード: corb, cnnm, duf21, magnesium transporter, metal transport, membrane protein
• 由来する生物種: Methanoculleus thermophilus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15830.18

構造登録者

Chen, Y.S.,Gehring, K. (登録日: 2021-05-12, 公開日: 2021-06-16, 最終更新日: 2023-10-18)

主引用文献

Chen, Y.S.,Kozlov, G.,Moeller, B.E.,Rohaim, A.,Fakih, R.,Roux, B.,Burke, J.E.,Gehring, K.
Crystal structure of an archaeal CorB magnesium transporter.
Nat Commun, 12:4028-4028, 2021

PubMed Abstract: CNNM/CorB proteins are a broadly conserved family of integral membrane proteins with close to 90,000 protein sequences known. They are associated with Mg transport but it is not known if they mediate transport themselves or regulate other transporters. Here, we determine the crystal structure of an archaeal CorB protein in two conformations (apo and Mg-ATP bound). The transmembrane DUF21 domain exists in an inward-facing conformation with a Mg ion coordinated by a conserved π-helix. In the absence of Mg-ATP, the CBS-pair domain adopts an elongated dimeric configuration with previously unobserved domain-domain contacts. Hydrogen-deuterium exchange mass spectrometry, analytical ultracentrifugation, and molecular dynamics experiments support a role of the structural rearrangements in mediating Mg-ATP sensing. Lastly, we use an in vitro, liposome-based assay to demonstrate direct Mg transport by CorB proteins. These structural and functional insights provide a framework for understanding function of CNNMs in Mg transport and associated diseases.

PubMed: 34188059
DOI: 10.1038/s41467-021-24282-7

実験手法

X-RAY DIFFRACTION (2.07 Å)