7MSL

Structure of anti-CRISPR AcrIIC4 from Haemophilus parainfluenzae

7MSL の概要

• エントリーDOI: 10.2210/pdb7msl/pdb
• 分子名称: AcrIIC4, IODIDE ION (3 entities in total)
• 機能のキーワード: anti-crispr proteins, acriic4, a haemophilus parainfluenzae prophage, viral protein
• 由来する生物種: Haemophilus parainfluenzae
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 10712.43

構造登録者

Pan, C.,Maxwell, K.L.,Moraes, T.F. (登録日: 2021-05-11, 公開日: 2022-03-23, 最終更新日: 2024-05-22)

主引用文献

Hwang, S.,Pan, C.,Garcia, B.,Davidson, A.R.,Moraes, T.F.,Maxwell, K.L.
Structural and Mechanistic Insight into CRISPR-Cas9 Inhibition by Anti-CRISPR Protein AcrIIC4 Hpa.
J.Mol.Biol., 434:167420-167420, 2022

PubMed Abstract: Phages, plasmids, and other mobile genetic elements express inhibitors of CRISPR-Cas immune systems, known as anti-CRISPR proteins, to protect themselves from targeted destruction. These anti-CRISPR proteins have been shown to function through very diverse mechanisms. In this work we investigate the activity of an anti-CRISPR isolated from a prophage in Haemophilus parainfluenzae that blocks CRISPR-Cas9 DNA cleavage activity. We determine the three-dimensional crystal structure of AcrIIC4 and show that it binds to the Cas9 Recognition Domain. This binding does not prevent the Cas9-anti-CRISPR complex from interacting with target DNA but does inhibit DNA cleavage. AcrIIC4 likely acts by blocking the conformational changes that allow the HNH and RuvC endonuclease domains to contact the DNA sites to be nicked.

PubMed: 34954237
DOI: 10.1016/j.jmb.2021.167420

実験手法

X-RAY DIFFRACTION (1.73 Å)