7MQ8

Cryo-EM structure of the human SSU processome, state pre-A1

これはPDB形式変換不可エントリーです。

7MQ8 の概要

• エントリーDOI: 10.2210/pdb7mq8/pdb
• EMDBエントリー: 23936 23937 23938 23939 23940
• 分子名称: 5'ETS rRNA, 40S ribosomal protein S9, 40S ribosomal protein S12, ... (76 entities in total)
• 機能のキーワード: ribosomal assembly intermediate, ribosome
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 76
• 化学式量合計: 5778246.34

構造登録者

Vanden Broeck, A.,Singh, S.,Klinge, S. (登録日: 2021-05-05, 公開日: 2021-09-22, 最終更新日: 2024-08-28)

主引用文献

Singh, S.,Vanden Broeck, A.,Miller, L.,Chaker-Margot, M.,Klinge, S.
Nucleolar maturation of the human small subunit processome.
Science, 373:eabj5338-eabj5338, 2021

PubMed Abstract: The human small subunit processome mediates early maturation of the small ribosomal subunit by coupling RNA folding to subsequent RNA cleavage and processing steps. We report the high-resolution cryo–electron microscopy structures of maturing human small subunit (SSU) processomes at resolutions of 2.7 to 3.9 angstroms. These structures reveal the molecular mechanisms that enable crucial progressions during SSU processome maturation. RNA folding states within these particles are communicated to and coordinated with key enzymes that drive irreversible steps such as targeted exosome-mediated RNA degradation, protein-guided site-specific endonucleolytic RNA cleavage, and tightly controlled RNA unwinding. These conserved mechanisms highlight the SSU processome’s impressive structural plasticity, which endows this 4.5-megadalton nucleolar assembly with the distinctive ability to mature the small ribosomal subunit from within.

PubMed: 34516797
DOI: 10.1126/science.abj5338

実験手法

ELECTRON MICROSCOPY (3.6 Å)