7MLL

Solution structure of Exenatide (exendin-4) in 30-vol% trifluoroethanol using CS-Rosetta

7MLL の概要

• エントリーDOI: 10.2210/pdb7mll/pdb
• NMR情報: BMRB: 50886
• 分子名称: Exendin-4 (1 entity in total)
• 機能のキーワード: anti-diabetic drug, signaling receptor binding, protein binding
• 由来する生物種: Heloderma suspectum (Gila monster)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 4189.60

構造登録者

Mishra, S.H.,Bhavaraju, S. (登録日: 2021-04-28, 公開日: 2021-05-19, 最終更新日: 2024-10-30)

主引用文献

Mishra, S.H.,Bhavaraju, S.,Schmidt, D.R.,Carrick, K.L.
Facilitated structure verification of the biopharmaceutical peptide exenatide by 2D heteronuclear NMR maps.
J Pharm Biomed Anal, 203:114136-114136, 2021

PubMed Abstract: Exenatide is a peptide based anti-diabetic prescription medication. Until now, the literature has lacked a comprehensive atom-specific molecular characterization for this complex large peptide by NMR spectroscopy that can be effortlessly and rapidly utilized for biopharmaceutical structural veracity. Peptide structure verification by NMR is challenging and cumbersome when reliant on traditional proton-based methodology (through-bond and through-space proton connectivity) alone due to increasing complexity, low signal dispersion, and overlap. These challenges are overcome by using 2D heteronuclear (H-C and H-N) maps that not only allow unambiguous signal assignment, but also condense the structural verification information within simplified peptide amide and carbon fingerprint maps. Here we report such simplified amide and carbon fingerprint maps for exenatide; made possible by the first ever comprehensive heteronuclear (H,C, and N) atom specific assignment of exenatide. These heteronuclear assignments were obtained without any isotopic enrichments i.e. at natural abundance, and hence are easily deployable as routine procedures. Furthermore, we compare the 2D heteronuclear maps of exenatide to a chemically identical peptide differing only in the isomerism of the Cα position of the first amino acid, [dHis1]-exenatide, to demonstrate the uniqueness of these maps. We show that despite deliberate changes in pH, temperature, and concentrations, the differences between the amide maps of exenatide and [dHis1]-exenatide are retained. The work presented here not only provides a facilitated structure verification of exenatide but also a framework for heteronuclear NMR data acquisition and signal assignment of large peptides, at natural abundance, in creating their respective unique 2D fingerprint maps.

PubMed: 34087552
DOI: 10.1016/j.jpba.2021.114136

実験手法

SOLUTION NMR