7MKF

Paired helical tau filament extracted from PrP-CAA Patient brain tissue | tau filament | PHF Tau

7MKF の概要

• エントリーDOI: 10.2210/pdb7mkf/pdb
• EMDBエントリー: 23871
• 分子名称: Isoform Tau-F of Microtubule-associated protein tau (1 entity in total)
• 機能のキーワード: tau filament, gss, prp-caa, amyloid fibril, alzheimer disease, protein fibril
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 459198.71

構造登録者

Hoq, M.R. (登録日: 2021-04-23, 公開日: 2021-07-28, 最終更新日: 2024-05-29)

主引用文献

Hallinan, G.I.,Hoq, M.R.,Ghosh, M.,Vago, F.S.,Fernandez, A.,Garringer, H.J.,Vidal, R.,Jiang, W.,Ghetti, B.
Structure of Tau filaments in Prion protein amyloidoses.
Acta Neuropathol, 142:227-241, 2021

PubMed Abstract: In human neurodegenerative diseases associated with the intracellular aggregation of Tau protein, the ordered cores of Tau filaments adopt distinct folds. Here, we analyze Tau filaments isolated from the brain of individuals affected by Prion-Protein cerebral amyloid angiopathy (PrP-CAA) with a nonsense mutation in the PRNP gene that leads to early termination of translation of PrP (Q160Ter or Q160X), and Gerstmann-Sträussler-Scheinker (GSS) disease, with a missense mutation in the PRNP gene that leads to an amino acid substitution at residue 198 (F198S) of PrP. The clinical and neuropathologic phenotypes associated with these two mutations in PRNP are different; however, the neuropathologic analyses of these two genetic variants have consistently shown the presence of numerous neurofibrillary tangles (NFTs) made of filamentous Tau aggregates in neurons. We report that Tau filaments in PrP-CAA (Q160X) and GSS (F198S) are composed of 3-repeat and 4-repeat Tau isoforms, having a striking similarity to NFTs in Alzheimer disease (AD). In PrP-CAA (Q160X), Tau filaments are made of both paired helical filaments (PHFs) and straight filaments (SFs), while in GSS (F198S), only PHFs were found. Mass spectrometry analyses of Tau filaments extracted from PrP-CAA (Q160X) and GSS (F198S) brains show the presence of post-translational modifications that are comparable to those seen in Tau aggregates from AD. Cryo-EM analysis reveals that the atomic models of the Tau filaments obtained from PrP-CAA (Q160X) and GSS (F198S) are identical to those of the Tau filaments from AD, and are therefore distinct from those of Pick disease, chronic traumatic encephalopathy, and corticobasal degeneration. Our data support the hypothesis that in the presence of extracellular amyloid deposits and regardless of the primary amino acid sequence of the amyloid protein, similar molecular mechanisms are at play in the formation of identical Tau filaments.

PubMed: 34128081
DOI: 10.1007/s00401-021-02336-w

実験手法

ELECTRON MICROSCOPY (3 Å)