7MKA

Structure of EC+EC (leading EC-focused)

7MKA の概要

• エントリーDOI: 10.2210/pdb7mka/pdb
• 関連するPDBエントリー: 7MEI
• EMDBエントリー: 23888
• 分子名称: DNA (40-MER), DNA-directed RNA polymerases I, II, and III subunit RPABC3, DNA-directed RNA polymerase II subunit RPB9, ... (17 entities in total)
• 機能のキーワード: pol ii, transcription
• 由来する生物種: Saccharomyces cerevisiae (Baker's yeast); 詳細
• タンパク質・核酸の鎖数: 15
• 化学式量合計: 544039.81

構造登録者

Yang, C.,Murakami, K. (登録日: 2021-04-22, 公開日: 2022-04-27, 最終更新日: 2024-10-30)

主引用文献

Yang, C.,Fujiwara, R.,Kim, H.J.,Basnet, P.,Zhu, Y.,Gorbea Colon, J.J.,Steimle, S.,Garcia, B.A.,Kaplan, C.D.,Murakami, K.
Structural visualization of de novo transcription initiation by Saccharomyces cerevisiae RNA polymerase II.
Mol.Cell, 82:660-676.e9, 2022

PubMed Abstract: Previous structural studies of the initiation-elongation transition of RNA polymerase II (pol II) transcription have relied on the use of synthetic oligonucleotides, often artificially discontinuous to capture pol II in the initiating state. Here, we report multiple structures of initiation complexes converted de novo from a 33-subunit yeast pre-initiation complex (PIC) through catalytic activities and subsequently stalled at different template positions. We determine that PICs in the initially transcribing complex (ITC) can synthesize a transcript of ∼26 nucleotides before transitioning to an elongation complex (EC) as determined by the loss of general transcription factors (GTFs). Unexpectedly, transition to an EC was greatly accelerated when an ITC encountered a downstream EC stalled at promoter proximal regions and resulted in a collided head-to-end dimeric EC complex. Our structural analysis reveals a dynamic state of TFIIH, the largest of GTFs, in PIC/ITC with distinct functional consequences at multiple steps on the pathway to elongation.

PubMed: 35051353
DOI: 10.1016/j.molcel.2021.12.020

実験手法

ELECTRON MICROSCOPY (3.54 Å)