7MIA

Maize rayado fino virus protease

7MIA の概要

• エントリーDOI: 10.2210/pdb7mia/pdb
• 分子名称: RNA replication protein (2 entities in total)
• 機能のキーワード: protease, deubiquitinase, pro replicase protein, viral protein, hydrolase
• 由来する生物種: Maize rayado fino virus (MRFV)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 32874.97

構造登録者

Patel, A.,Mark, B.L. (登録日: 2021-04-16, 公開日: 2021-07-21, 最終更新日: 2023-10-18)

主引用文献

Patel, A.,McBride, J.A.M.,Mark, B.L.
The endopeptidase of the maize-affecting Marafivirus type member maize rayado fino virus doubles as a deubiquitinase.
J.Biol.Chem., 297:100957-100957, 2021

PubMed Abstract: Marafiviruses are capable of persistent infection in a range of plants that have importance to the agriculture and biofuel industries. Although the genomes of a few of these viruses have been studied in-depth, the composition and processing of the polyproteins produced from their main ORFs have not. The Marafivirus polyprotein consists of essential proteins that form the viral replicase, as well as structural proteins for virus assembly. It has been proposed that Marafiviruses code for cysteine proteases within their polyproteins, which act as endopeptidases to autocatalytically cleave the polyprotein into functional domains. Furthermore, it has also been suggested that Marafivirus endopeptidases may have deubiquitinating activity, which has been shown to enhance viral replication by downregulating viral protein degradation by the ubiquitin (Ub) proteasomal pathway as well as tampering with cell signaling associated with innate antiviral responses in other positive-sense ssRNA viruses. Here, we provide the first evidence of cysteine proteases from six different Marafiviruses that harbor deubiquitinating activity and reveal intragenus differences toward Ub linkage types. We also examine the structural basis of the endopeptidase/deubiquitinase from the Marafivirus type member, maize rayado fino virus. Structures of the enzyme alone and bound to Ub reveal marked structural rearrangements that occur upon binding of Ub and provide insights into substrate specificity and differences that set it apart from other viral cysteine proteases.

PubMed: 34265303
DOI: 10.1016/j.jbc.2021.100957

実験手法

X-RAY DIFFRACTION (1.9 Å)