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7MI2

Seb1-G476S RNA binding domain

Summary for 7MI2
Entry DOI10.2210/pdb7mi2/pdb
DescriptorRpb7-binding protein seb1, GLYCEROL, DI(HYDROXYETHYL)ETHER, ... (4 entities in total)
Functional Keywordsrrm, termination factor, transcription interference, rna binding protein
Biological sourceSchizosaccharomyces pombe (Fission yeast)
Total number of polymer chains1
Total formula weight17251.65
Authors
Jacewicz, A.,Shuman, S. (deposition date: 2021-04-16, release date: 2021-07-14, Last modification date: 2023-10-18)
Primary citationSchwer, B.,Garg, A.,Jacewicz, A.,Shuman, S.
Genetic screen for suppression of transcriptional interference identifies a gain-of-function mutation in Pol2 termination factor Seb1.
Proc.Natl.Acad.Sci.USA, 118:-, 2021
Cited by
PubMed Abstract: The system of long noncoding RNA (lncRNA)-mediated transcriptional interference that represses fission yeast phosphate homoeostasis gene provides a sensitive readout of genetic influences on cotranscriptional 3'-processing and termination and a tool for discovery of regulators of this phase of the Pol2 transcription cycle. Here, we conducted a genetic screen for relief of transcriptional interference that unveiled a mechanism by which Pol2 termination is enhanced via a gain-of-function mutation, G476S, in the RNA-binding domain of an essential termination factor, Seb1. The genetic and physical evidence for gain-of-function is compelling: 1) de-represses and , both of which are subject to lncRNA-mediated transcriptional interference; 2) elicits precocious lncRNA transcription termination in response to lncRNA 5'-proximal poly(A) signals; 3) derepression of is effaced by loss-of-function mutations in cleavage and polyadenylation factor (CPF) subunits and termination factor Rhn1; 4) synthetic lethality of with derepressive mutants and ∆ is rescued by CPF/Rhn1 loss-of-function alleles; and 5) elicits an upstream shift in poly(A) site preference in several messenger RNA genes. A crystal structure of the Seb1-G476S RNA-binding domain indicates potential for gain of contacts from Ser476 to RNA nucleobases. To our knowledge, this is a unique instance of a gain-of-function phenotype in a eukaryal transcription termination protein.
PubMed: 34389684
DOI: 10.1073/pnas.2108105118
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (1.4 Å)
Structure validation

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건을2024-11-06부터공개중

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