7MI2

Seb1-G476S RNA binding domain

7MI2 の概要

• エントリーDOI: 10.2210/pdb7mi2/pdb
• 分子名称: Rpb7-binding protein seb1, GLYCEROL, DI(HYDROXYETHYL)ETHER, ... (4 entities in total)
• 機能のキーワード: rrm, termination factor, transcription interference, rna binding protein
• 由来する生物種: Schizosaccharomyces pombe (Fission yeast)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 17251.65

構造登録者

Jacewicz, A.,Shuman, S. (登録日: 2021-04-16, 公開日: 2021-07-14, 最終更新日: 2023-10-18)

主引用文献

Schwer, B.,Garg, A.,Jacewicz, A.,Shuman, S.
Genetic screen for suppression of transcriptional interference identifies a gain-of-function mutation in Pol2 termination factor Seb1.
Proc.Natl.Acad.Sci.USA, 118:-, 2021

PubMed Abstract: The system of long noncoding RNA (lncRNA)-mediated transcriptional interference that represses fission yeast phosphate homoeostasis gene provides a sensitive readout of genetic influences on cotranscriptional 3'-processing and termination and a tool for discovery of regulators of this phase of the Pol2 transcription cycle. Here, we conducted a genetic screen for relief of transcriptional interference that unveiled a mechanism by which Pol2 termination is enhanced via a gain-of-function mutation, G476S, in the RNA-binding domain of an essential termination factor, Seb1. The genetic and physical evidence for gain-of-function is compelling: 1) de-represses and , both of which are subject to lncRNA-mediated transcriptional interference; 2) elicits precocious lncRNA transcription termination in response to lncRNA 5'-proximal poly(A) signals; 3) derepression of is effaced by loss-of-function mutations in cleavage and polyadenylation factor (CPF) subunits and termination factor Rhn1; 4) synthetic lethality of with derepressive mutants and ∆ is rescued by CPF/Rhn1 loss-of-function alleles; and 5) elicits an upstream shift in poly(A) site preference in several messenger RNA genes. A crystal structure of the Seb1-G476S RNA-binding domain indicates potential for gain of contacts from Ser476 to RNA nucleobases. To our knowledge, this is a unique instance of a gain-of-function phenotype in a eukaryal transcription termination protein.

PubMed: 34389684
DOI: 10.1073/pnas.2108105118

実験手法

X-RAY DIFFRACTION (1.4 Å)