7MF3

Structure of the autoinhibited state of smooth muscle myosin-2

7MF3 の概要

• エントリーDOI: 10.2210/pdb7mf3/pdb
• EMDBエントリー: 23810
• 分子名称: Myosin-11, Myosin light polypeptide 6, Myosin regulatory light chain 2, smooth muscle major isoform, ... (6 entities in total)
• 機能のキーワード: muscle contraction, atpase, autoinhibition, 10s, contractile protein
• 由来する生物種: Gallus gallus (Chicken); 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 990445.52

構造登録者

Heissler, S.M.,Arora, A.S.,Billington, N.,Sellers, J.R.,Chinthalapudi, K. (登録日: 2021-04-08, 公開日: 2022-01-05, 最終更新日: 2024-05-29)

主引用文献

Heissler, S.M.,Arora, A.S.,Billington, N.,Sellers, J.R.,Chinthalapudi, K.
Cryo-EM structure of the autoinhibited state of myosin-2.
Sci Adv, 7:eabk3273-eabk3273, 2021

PubMed Abstract: We solved the near-atomic resolution structure of smooth muscle myosin-2 in the autoinhibited state (10) using single-particle cryo–electron microscopy. The 3.4-Å structure reveals the precise molecular architecture of 10 and the structural basis for myosin-2 regulation. We reveal the position of the phosphorylation sites that control myosin autoinhibition and activation by phosphorylation of the regulatory light chain. Further, we present a previously unidentified conformational state in myosin-2 that traps ADP and P produced by the hydrolysis of ATP in the active site. This noncanonical state represents a branch of the myosin enzyme cycle and explains the autoinhibition of the enzyme function of 10 along with its reduced affinity for actin. Together, our structure defines the molecular mechanisms that drive 10 formation, stabilization, and relief by phosphorylation of the regulatory light chain.

PubMed: 34936462
DOI: 10.1126/sciadv.abk3273

実験手法

ELECTRON MICROSCOPY (3.4 Å)