7MDS

Crystal structure of AtDHDPS1 in complex with MBDTA-2

7MDS の概要

• エントリーDOI: 10.2210/pdb7mds/pdb
• 分子名称: 4-hydroxy-tetrahydrodipicolinate synthase 1, chloroplastic, {(5Z)-5-[(4-methoxyphenyl)methylidene]-2,4-dioxo-1,3-thiazolidin-3-yl}acetic acid, CHLORIDE ION, ... (4 entities in total)
• 機能のキーワード: herbicide, lysine biosynthesis, biosynthetic protein, lyase-lyase inhibitor complex, lyase/lyase inhibitor
• 由来する生物種: Arabidopsis thaliana (Mouse-ear cress)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 76446.94

構造登録者

Hall, C.J.,Soares da Costa, T.P.,Panjikar, S. (登録日: 2021-04-06, 公開日: 2021-08-04, 最終更新日: 2024-11-06)

主引用文献

Soares da Costa, T.P.,Hall, C.J.,Panjikar, S.,Wyllie, J.A.,Christoff, R.M.,Bayat, S.,Hulett, M.D.,Abbott, B.M.,Gendall, A.R.,Perugini, M.A.
Towards novel herbicide modes of action by inhibiting lysine biosynthesis in plants.
Elife, 10:-, 2021

PubMed Abstract: Weeds are becoming increasingly resistant to our current herbicides, posing a significant threat to agricultural production. Therefore, new herbicides with novel modes of action are urgently needed. In this study, we exploited a novel herbicide target, dihydrodipicolinate synthase (DHDPS), which catalyses the first and rate-limiting step in lysine biosynthesis. The first class of plant DHDPS inhibitors with micromolar potency against DHDPS was identified using a high-throughput chemical screen. We determined that this class of inhibitors binds to a novel and unexplored pocket within DHDPS, which is highly conserved across plant species. The inhibitors also attenuated the germination and growth of seedlings and confirmed their pre-emergence herbicidal activity in soil-grown plants. These results provide proof-of-concept that lysine biosynthesis represents a promising target for the development of herbicides with a novel mode of action to tackle the global rise of herbicide-resistant weeds.

PubMed: 34313586
DOI: 10.7554/eLife.69444

実験手法

X-RAY DIFFRACTION (2.295 Å)