7MCA

Structure of the S. cerevisiae origin recognition complex bound to the replication initiator Cdc6 and the ARS1 origin DNA.

7MCA の概要

• エントリーDOI: 10.2210/pdb7mca/pdb
• EMDBエントリー: 23755 23818
• 分子名称: Origin recognition complex subunit 1, MAGNESIUM ION, PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER, ... (11 entities in total)
• 機能のキーワード: replication initiation, replication
• 由来する生物種: Saccharomyces cerevisiae (Baker's yeast); 詳細
• タンパク質・核酸の鎖数: 9
• 化学式量合計: 527256.28

構造登録者

Feng, X.,Li, H. (登録日: 2021-04-01, 公開日: 2021-06-02, 最終更新日: 2024-05-29)

主引用文献

Feng, X.,Noguchi, Y.,Barbon, M.,Stillman, B.,Speck, C.,Li, H.
The structure of ORC-Cdc6 on an origin DNA reveals the mechanism of ORC activation by the replication initiator Cdc6.
Nat Commun, 12:3883-3883, 2021

PubMed Abstract: The Origin Recognition Complex (ORC) binds to sites in chromosomes to specify the location of origins of DNA replication. The S. cerevisiae ORC binds to specific DNA sequences throughout the cell cycle but becomes active only when it binds to the replication initiator Cdc6. It has been unclear at the molecular level how Cdc6 activates ORC, converting it to an active recruiter of the Mcm2-7 hexamer, the core of the replicative helicase. Here we report the cryo-EM structure at 3.3 Å resolution of the yeast ORC-Cdc6 bound to an 85-bp ARS1 origin DNA. The structure reveals that Cdc6 contributes to origin DNA recognition via its winged helix domain (WHD) and its initiator-specific motif. Cdc6 binding rearranges a short α-helix in the Orc1 AAA+ domain and the Orc2 WHD, leading to the activation of the Cdc6 ATPase and the formation of the three sites for the recruitment of Mcm2-7, none of which are present in ORC alone. The results illuminate the molecular mechanism of a critical biochemical step in the licensing of eukaryotic replication origins.

PubMed: 34162887
DOI: 10.1038/s41467-021-24199-1

実験手法

ELECTRON MICROSCOPY (3.6 Å)