7M6U

Crystal structure of a circular permutation and computationally designed pro-enzyme of carboxypeptidase G2

Summary for 7M6U

• Entry DOI: 10.2210/pdb7m6u/pdb
• Related: 1CG2 6XJ5
• Descriptor: Carboxypeptidase G2 circular permuation pro-domain fusion, ZINC ION, SULFATE ION, ... (4 entities in total)
• Functional Keywords: pro-enzyme, enzyme design, directed enzyme-prodrug therapy, methotrexate, circular permutation, hydrolase
• Biological source: Pseudomonas sp. (strain RS-16); More
• Total number of polymer chains: 4
• Total formula weight: 190273.35

Authors

Yachnin, B.J.,Khare, S.D. (deposition date: 2021-03-26, release date: 2021-04-14, Last modification date: 2023-10-18)

• Primary citation: Yachnin, B.J.,Azouz, L.R.,White 3rd, R.E.,Minetti, C.A.S.A.,Remeta, D.P.,Tan, V.M.,Drake, J.M.,Khare, S.D.; Massively parallel, computationally guided design of a proenzyme.; Proc.Natl.Acad.Sci.USA, 119:e2116097119-e2116097119, 2022; PubMed: 35377786; DOI: 10.1073/pnas.2116097119

Experimental method

X-RAY DIFFRACTION (2.59 Å)