7LU6

Crystal structure of the mouse Kirrel3 D1 homodimer

Summary for 7LU6

• Entry DOI: 10.2210/pdb7lu6/pdb
• Related: 7LTW
• Descriptor: Kin of IRRE-like protein 3, SODIUM ION (3 entities in total)
• Functional Keywords: immunoglobulin superfamily, cell surface receptor, nervous system, cell adhesion
• Biological source: Mus musculus (Mouse)
• Total number of polymer chains: 8
• Total formula weight: 97421.78

Authors

Roman, C.A.,Pak, J.S.,Wang, J.,Ozkan, E. (deposition date: 2021-02-21, release date: 2021-11-24, Last modification date: 2024-10-23)

Primary citation

Wang, J.,Vaddadi, N.,Pak, J.S.,Park, Y.,Quilez, S.,Roman, C.A.,Dumontier, E.,Thornton, J.W.,Cloutier, J.F.,Ozkan, E.
Molecular and structural basis of olfactory sensory neuron axon coalescence by Kirrel receptors.
Cell Rep, 37:109940-109940, 2021

PubMed Abstract: Projections from sensory neurons of olfactory systems coalesce into glomeruli in the brain. The Kirrel receptors are believed to homodimerize via their ectodomains and help separate sensory neuron axons into Kirrel2- or Kirrel3-expressing glomeruli. Here, we present the crystal structures of homodimeric Kirrel receptors and show that the closely related Kirrel2 and Kirrel3 have evolved specific sets of polar and hydrophobic interactions, respectively, disallowing heterodimerization while preserving homodimerization, likely resulting in proper segregation and coalescence of Kirrel-expressing axons into glomeruli. We show that the dimerization interface at the N-terminal immunoglobulin (IG) domains is necessary and sufficient to create homodimers and fail to find evidence for a secondary interaction site in Kirrel ectodomains. Furthermore, we show that abolishing dimerization of Kirrel3 in vivo leads to improper formation of glomeruli in the mouse accessory olfactory bulb as observed in Kirrel3 animals. Our results provide evidence for Kirrel3 homodimerization controlling axonal coalescence.

PubMed: 34731636
DOI: 10.1016/j.celrep.2021.109940

Experimental method

X-RAY DIFFRACTION (1.95 Å)