7LS9

Cryo-EM structure of neutralizing antibody 1-57 in complex with prefusion SARS-CoV-2 spike glycoprotein

7LS9 の概要

• エントリーDOI: 10.2210/pdb7ls9/pdb
• EMDBエントリー: 23506
• 分子名称: Spike glycoprotein, 1-57 Fab heavy chain, 1-57 Fab light chain, ... (6 entities in total)
• 機能のキーワード: neutralizing antibody, fusion protein, spike glycoprotein, covid-19, rbd, rbd-directed antibody, 1-57, viral protein, immune system, viral protein-immune system complex, viral protein/immune system
• 由来する生物種: Severe acute respiratory syndrome coronavirus 2 (2019-nCoV); 詳細
• タンパク質・核酸の鎖数: 9
• 化学式量合計: 592526.31

構造登録者

Cerutti, G.,Shapiro, L. (登録日: 2021-02-17, 公開日: 2021-03-17, 最終更新日: 2024-10-09)

主引用文献

Cerutti, G.,Rapp, M.,Guo, Y.,Bahna, F.,Bimela, J.,Reddem, E.R.,Yu, J.,Wang, P.,Liu, L.,Huang, Y.,Ho, D.D.,Kwong, P.D.,Sheng, Z.,Shapiro, L.
Structural basis for accommodation of emerging B.1.351 and B.1.1.7 variants by two potent SARS-CoV-2 neutralizing antibodies.
Structure, 29:655-663.e4, 2021

PubMed Abstract: Emerging SARS-CoV-2 strains, B.1.1.7 and B.1.351, from the UK and South Africa, respectively, show decreased neutralization by monoclonal antibodies and convalescent or vaccinee sera raised against the original wild-type virus, and are thus of clinical concern. However, the neutralization potency of two antibodies, 1-57 and 2-7, which target the receptor-binding domain (RBD) of the spike, was unaffected by these emerging strains. Here, we report cryo-EM structures of 1-57 and 2-7 in complex with spike, revealing each of these antibodies to utilize a distinct mechanism to bypass or accommodate RBD mutations. Notably, each antibody represented an immune response with recognition distinct from those of frequent antibody classes. Moreover, many epitope residues recognized by 1-57 and 2-7 were outside hotspots of evolutionary pressure for ACE2 binding and neutralizing antibody escape. We suggest the therapeutic use of antibodies, such as 1-57 and 2-7, which target less prevalent epitopes, could ameliorate issues of monoclonal antibody escape.

PubMed: 34111408
DOI: 10.1016/j.str.2021.05.014

実験手法

ELECTRON MICROSCOPY (3.42 Å)