7LQM

Glucosamie-6-phosphate Deaminase from Pasturella multocida

7LQM の概要

• エントリーDOI: 10.2210/pdb7lqm/pdb
• 分子名称: Glucosamine-6-phosphate deaminase, 1,2-ETHANEDIOL (3 entities in total)
• 機能のキーワード: deaminase, nagb, sialic acid, hydrolase
• 由来する生物種: Pasteurella multocida (strain Pm70)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 129519.66

構造登録者

Subramanian, R.,Srinivasachari, S. (登録日: 2021-02-14, 公開日: 2022-01-19, 最終更新日: 2023-10-18)

主引用文献

Srinivasachari, S.,Tiwari, V.R.,Kharbanda, T.,Sowdamini, R.,Subramanian, R.
A dimer between monomers and hexamers-Oligomeric variations in glucosamine-6-phosphate deaminase family.
Plos One, 18:e0271654-e0271654, 2023

PubMed Abstract: In bacteria that live in hosts whose terminal sugar is a sialic acid, Glucosamine-6-phosphate deaminase (NagB) catalyzes the last step in converting sialic acid into Fructose-6-phosphate. These bacteria then use the Fructose-6-phosphate as an energy source. The enzyme NagB exists as a hexamer in Gram-negative bacteria and is allosterically regulated. In Gram-positive bacteria, it exists as a monomer and lacks allosteric regulation. Our identification of a dimeric Gram-negative bacterial NagB motivated us to characterize the structural basis of two closely related oligomeric forms. We report here the crystal structures of NagB from two Gram-negative pathogens, Haemophilus influenzae (Hi) and Pasturella multocida (Pm). The Hi-NagB is active as a hexamer, while Pm-NagB is active as a dimer. Both Hi-NagB and Pm-NagB contain the C-terminal helix implicated as essential for hexamer formation. The hexamer is described as a dimer of trimers. In the Pm-NagB dimer, the dimeric interface is conserved. The conservation of the dimer interface suggests that the three possible oligomeric forms of NagB are a monomer, a dimer, and a trimer of dimers. Computational modeling and MD simulations indicate that the residues at the trimeric interface have less stabilizing energy of oligomer formation than those in the dimer interface. We propose that Pm-NagB is the evolutionary link between the monomer and the hexamer forms.

PubMed: 36598911
DOI: 10.1371/journal.pone.0271654

実験手法

X-RAY DIFFRACTION (2.3 Å)