7LO0

Structure of human ASF1a in complex with a TLK2 peptide

7LO0 の概要

• エントリーDOI: 10.2210/pdb7lo0/pdb
• 分子名称: Histone chaperone ASF1A, Serine/threonine-protein kinase tousled-like 2 (3 entities in total)
• 機能のキーワード: histone chaperone, kinase, docking, nucleosome assembly, cell cycle
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 24
• 化学式量合計: 182635.60

構造登録者

Simon, B.,Calderwood, D.,Turk, B.E.,Boggon, T.J. (登録日: 2021-02-08, 公開日: 2022-02-16, 最終更新日: 2023-10-18)

主引用文献

Simon, B.,Lou, H.J.,Huet-Calderwood, C.,Shi, G.,Boggon, T.J.,Turk, B.E.,Calderwood, D.A.
Tousled-like kinase 2 targets ASF1 histone chaperones through client mimicry.
Nat Commun, 13:749-749, 2022

PubMed Abstract: Tousled-like kinases (TLKs) are nuclear serine-threonine kinases essential for genome maintenance and proper cell division in animals and plants. A major function of TLKs is to phosphorylate the histone chaperone proteins ASF1a and ASF1b to facilitate DNA replication-coupled nucleosome assembly, but how TLKs selectively target these critical substrates is unknown. Here, we show that TLK2 selectivity towards ASF1 substrates is achieved in two ways. First, the TLK2 catalytic domain recognizes consensus phosphorylation site motifs in the ASF1 C-terminal tail. Second, a short sequence at the TLK2 N-terminus docks onto the ASF1a globular N-terminal domain in a manner that mimics its histone H3 client. Disrupting either catalytic or non-catalytic interactions through mutagenesis hampers ASF1 phosphorylation by TLK2 and cell growth. Our results suggest that the stringent selectivity of TLKs for ASF1 is enforced by an unusual interaction mode involving mutual recognition of a short sequence motifs by both kinase and substrate.

PubMed: 35136069
DOI: 10.1038/s41467-022-28427-0

実験手法

X-RAY DIFFRACTION (2.71 Å)